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Recombinant TransformationPlasmid Digest. Obtain four 1.5 ml reagent tubes labeled: - pAmp - pKan - 2XRB"(Restriction Buffer) - B/H" (BamHI and HindIII enzymes).. Use the matrix below as a checklist while adding reagents to the pAmp and pKan tube. Use a fresh pipette tip for each reagent. Add all reagents directly to the solution..
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