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Marketa 9-18-07 Ongoing projects: 1. AuNP Formation – 1:4 - time study completed

Marketa 9-18-07 Ongoing projects: 1. AuNP Formation – 1:4 - time study completed 1:1 - time study completed, 4:1 - time study completed, TEM 2. GEPI-Directed Immobilization of Biomolecules-MANUSCIPT

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Marketa 9-18-07 Ongoing projects: 1. AuNP Formation – 1:4 - time study completed

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  1. Marketa 9-18-07 • Ongoing projects: • 1. AuNP Formation – • 1:4 - time study completed • 1:1 - time study completed, • 4:1 - time study completed, TEM • 2. GEPI-Directed Immobilization of Biomolecules-MANUSCIPT • AFM of AuBP2L and QBP1-bio + streptavidinQD on NSL samples (proof of principle AuBP can be used as linker for LSPR studies) • LSPR of AuBP2L-bio (probe) and streptavidin (target) using new approach (Turgay) • 3. Thiol experiment on Au/Ag pattern substrates • - 30:70 and 50:50 thiol:thiol-biotin –as a positive control for AuBP’s cross-specificity exp. - in progress • 4. Aragonite project • - sequences from round#3 clones - completed • FM of clones from round#3 – completed • PCR of clones from round#4 – completed • FM of clones from round#4 – completed • 5. Flagella Project : Genetically Engineered Flagella as a Scaffold for Au Formation • 6. ADP binding experiment – FM experiment using biotinylated peptides and streptavidin QD

  2. 9-12-07 20x ADP1 ADP2 0.1mg HA powder sigma 3hr incubation 56uM peptide 15min incubation 4nM strepQD

  3. 9-12-07 20x ADP4 ADP6 0.1mg HA powder sigma 3hr incubation 56uM peptide 15min incubation 4nM strepQD

  4. 9-12-07 20x ADP7 Blank NC 0.1mg HA powder sigma 3hr incubation 56uM peptide 15min incubation 4nM strepQD

  5. Aragonite-binding sequences - CSD - Flitrx Library - 4 selection round - Last round (Round#4) – “counter selection with calcite”

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