TEDx San Diego – Chain Reaction

Apr 17, 2019

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I had the pleasure of sketchnoting TEDx San Diego.

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TEDx San Diego – Chain Reaction

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Polymerase Chain Reaction - PCR

Polymerase Chain Reaction - PCR. The polymerase chain reaction (PCR) is a technique to amplify a piece of DNA very rapidly outside of a cell. Some applications of PCR. Forensic medicine. Preimplantation Genetic Diagnosis (PGD). Archeology. Paternity testing.

666 views • 17 slides

HOFMANN REACTION

HOFMANN REACTION. E2 gone astray. HOFMANN RULE. When you have a bulky leaving group like -N(CH 3 ) 3 + the least-substituted alkene will be the major product. Big is not the same as bulky. BULKY =. Branched at the first atom attached to the chain. chain. chain. trimethyl

1.91k views • 33 slides

23.2 Explosions

23.2 Explosions. Thermal explosion: a very rapid reaction arising from a rapid increase of reaction rate with increasing temperature. Chain-branching explosion: occurs when the number of chain centres grows exponentially. An example of both types of explosion is the following reaction

1.29k views • 16 slides

Polymerase Chain Reaction

Polymerase Chain Reaction. a.k.a. “How’d they get all that DNA from just a little blood?”. What does PCR stand for?. Polymerase Chain Reaction Developed by Kary Mullis – Nobel Prize Received a $20,000 bonus; later sold it to Hoffman-LaRoche for $300,000,000. What is the goal of PCR?.

1.54k views • 41 slides

Polymerase Chain Reaction

Investigation strategies and methods. Polymerase Chain Reaction. May 2007. Learning objectives. At the end of the presentation, participants should know: History of polymerase chain reaction (PCR) Definition and short technical overview of PCR Applications of PCR Restrictions of PCR

897 views • 24 slides

Chain Reaction Mechanisms

Chain Reaction Mechanisms - cont'd.. Initiation: A step in which a stable molecular species (not necessarily a reactant in the overall reaction) reacts to generate one or more reactive intermediatesPropagation: A reactive intermediate attacks a reactant in the overall reaction to generate a new r

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Polymerase Chain Reaction (PCR) and Its Applications

Polymerase Chain Reaction (PCR) and Its Applications. by Ayaz Najafov Boğaziçi University Department of Molecular Biology and Genetics. What is PCR?. PCR is an exponentially progressing synthesis of the defined target DNA sequences in vitro .

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The Polymerase Chain Reaction

The Polymerase Chain Reaction. Chapter 6: Background. Invention of PCR. Kary Mullis Mile marker 46.58 in April of 1983 Pulled off the road and outlined a way to conduct DNA replication in a tube Worked for Cetus, which was purchased by Chiron and is now part of Novartis. What is PCR?.

865 views • 38 slides

Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990 Apr;262(4):56-61, 64-5

Mullis KB. The unusual origin of the polymerase chain reaction. Sci Am. 1990 Apr;262(4):56-61, 64-5. What is the Polymerase Chain Reaction?. Template: Base-paired dsDNA. 3’. 5’. 3’. 5’. What is the Polymerase Chain Reaction?. 1. Heat Denature. What is the Polymerase Chain Reaction?.

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Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR). Julie McDonald Alli Hicks Courtney Dailey . Polymerase Chain Reaction. How is it used? Medical and biological research for a variety of applications

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Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR). Used to amplify a sample of DNA Many applications, including: DNA sequencing Phylogeny Diagnosis of genetic disorders Identity for forensics or paternity Amplification is exponential Requires thermal cycling DNA is doubled each cycle

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Polymerase Chain Reaction

PCR. Polymerase Chain Reaction. (PCR). PCR. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone) Allows scientists to isolate pure quantities of specific DNA sequences

274 views • 15 slides

Polymerase Chain Reaction

Polymerase Chain Reaction. PCR. (PCR). P C R. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone) Allows scientists to isolate pure quantities of specific DNA sequences

323 views • 19 slides

Polymerase Chain Reaction

Polymerase Chain Reaction. Qai Gordon and Maddy Marchetti. What is Polymerase Chain Reaction?. Polymerase Chain Reaction ( PCR ) is a process that amplifies small pieces of DNA to create multiple copies of that DNA sequence Also called DNA amplification. Applications of PCR.

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Polymerase Chain Reaction (basic principal)

Polymerase Chain Reaction (basic principal). Polymerase Chain Reaction (PCR). Developed by Kary Mullis and described in 1985 (Saiki et al., 1985) Mimics repeated cycles of DNA replication to amplify specific sequences of DNA Subsequently modified to use RNA as the template for replication.

1.02k views • 75 slides

PCR POLYMERASE CHAIN REACTION

PCR POLYMERASE CHAIN REACTION. Dr. Venkateswaran A. PCR. Not a discovery – It is an Invention It is in one line – Making millions of copies of a DNA fragment Kary Mullis - inventor of pcr – won Nobel prize in 1993

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Polymerase Chain Reaction (PCR) and Its Applications

Polymerase Chain Reaction (PCR) and Its Applications. by Ayaz Najafov. What is PCR?. PCR is an exponentially progressing synthesis of the defined target DNA sequences in vitro. It was invented in 1983 by Dr. Kary Mullis, for which he received the Nobel Prize in Chemistry in 1993.

531 views • 32 slides

Polymerase Chain Reaction

Polymerase Chain Reaction. A process used to artificially multiply a chosen piece of genetic material. May also be known as DNA amplification. One strand of DNA may yield 2 30 strands or more. Uses of PCR. DNA sequencing. Gene cloning. DNA profiling. Transformation.

372 views • 16 slides

Mechanism of Some Typical Complex Reactions

Mechanism of Some Typical Complex Reactions. 1 Chain Reaction. §11.9. 1.1 basic concepts of chain reaction:. chain-initiating step. chain-propagating steps. chain-terminating step.

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POLIMERASE CHAIN REACTION

POLIMERASE CHAIN REACTION. Juanita Salgado Nicolás Borrero Nicolás Jaramillo. P.C.R. It is a technique used to produce large quantities of replicated DNA.

211 views • 9 slides

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