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Yin-Yang Column for Comprehensive Protein Identification

The Yin-Yang column offers a novel approach to protein identification through multi-LC/MS/MS, combining anion and cation exchange chromatography. Advantages include three-dimensional LC-MS/MS, complete coverage of peptides and proteins, and high sensitivity for phosphopeptides. With complementary methods like SCX and ANX, it can enrich and identify phospho and non-phospho peptides simultaneously. Theoretical pI distribution of non-phosphorylated peptides is illustrated, and the technology enables unique peptide identification and protein coverage. In a study with mouse liver protein digests, more than 1000 phosphopeptides were identified with minimal false positive rate.

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Yin-Yang Column for Comprehensive Protein Identification

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  1. Yin-Yang Column for Global Protein Identification Column Technology Inc.

  2. The Yin-Yang column combines anion and cation exchange chromatography For the protein identification through multi-LC/MS/MS: Yin-Yang MDLC-MS/MS

  3. Advantages of Yin-yang MDLC-MS/MS • Three-dimensional LC-MS/MS • SCX and ANX are complimentary methods for peptide fractionation • Most complete coverage of peptides and proteins • Enrichment and high-sensitivity for phosphopeptides • Identify phospho and non-phospho peptides in the same run.

  4. Theoretical pI distribution of identified non-phosphrylated peptides Complementary peptide identification of SCX and SAX

  5. 108413 Unique peptides SAX SCX 3047 9094 10254 6184 Proteins SCX SAX 2040 4152 4072 only 1mg protein digests of mouse liver, 5% FPR Another More than 1000 phosphopeptides identified

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