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Should I Drink The Water?

Should I Drink The Water?. Chris Azar Tony Rainsberger. Problem Description. Every year thousands of people get sick from drinking water when on vacation out of the country

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Should I Drink The Water?

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  1. Should I Drink The Water? Chris Azar Tony Rainsberger

  2. Problem Description • Every year thousands of people get sick from drinking water when on vacation out of the country • Vacations are ruined by symptoms which can include fever, vomiting, diarrhea, abdominal discomfort, bloating, and flatulence among others • Significant resources (time and money) are spent on medical treatment for avoidable conditions • Significant costs in the form of travel insurance payouts are incurred as a of these infections

  3. What if you could tell immediately, BEFORE you drink the water in a foreign country, if it is safe to drink? • Could you know from day 1 of your vacation if you could drink tap water or if you need to buy bottled water? • Could you enjoy your vacation without guessing about the safety of the water? • Could you possibly avoid the significant cost of having to buy bottled water or the considerably more significant cost of medical care?

  4. With pGLO Water Tester, Now You Can!! • pGLO water tester will quickly tell you if the water you are about to drink is contaminated with one, two, or all three of these common pathogens which can make you sick: • Giardia lamblia(a Protozoa which is the most common intestinal parasite) • Shigelladysenteriae(a bacterium which causes dysentery) • Hepatitis A (a virus which infects the liver)

  5. How Does pGLO Water Tester Work? • All three of these pathogens produce antigens (unique proteins) which are present in infected water • pGLO Water Tester can detect and respond to each or any combination of these antigens indicating if your water sample is contaminated. • The unique pGLO Water Test Plasmid is constructed to cause the expression of three different colored fluorescent proteins when exposed to the three different antigents as follows: • Giardia lambliawill produce a yellow fluorescent protein • Shigelladysenteriaewill produce a red fluorescent protein • Hepatitis A Virus will produce a blue fluorescent protein

  6. pGLO Water Tester Plasmid Design • Plasmid Components • Beta-lactamase: provides resistance to antibiotic (Ampicilin) • Antigen Receptor Genes – turns on the expression of the corresponding fluorescent protein gene. • Fluorescent Protein Gene – expression of these genes confirm the presence of any combination of these three pathogens

  7. If your water sample is contaminated, when you add pGLO Water Tester to it and expose it to UV light, it will glow. • The color that it glows will tell you what it is infected with according to the following chart:

  8. Further Implications • Wider Range of Pathogens • Sampling Systems • Test Strips • Air sampling • Commercial Treatment

  9. Work Cited • BioRad. (2012). pGLO Bacterial Transformation Kit. Retrieved from BioRad: http://www.bio-rad.com/prd/en/US/adirect/biorad?cmd=BRCatgProductDetail&isFromSearch=true&entryPoint=adirect&catID=619b8f74-9d3f-4c2f-a795-8a27e67598b7&vertical=LSE&messageType=BRCatgProductDetail&parentCategoryGUID=f75948d2-dc20-4a32-b4e5-b7e0fe4c21ed • CDC. (2009, November 16). Shigellosis. Retrieved from CDC: http://www.cdc.gov/nczved/divisions/dfbmd/diseases/shigellosis/ • CDC. (2011, March 8). Parasites - Giardia. Retrieved from CDC: http://www.cdc.gov/parasites/giardia/ • Pub Med. (2011, October 16). Hepatitis A. Retrieved from Pub Med Health: http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001323/

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