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Nicholas lab: C. elegans research

Nicholas lab: C. elegans research. Working with worms Postgraduate induction 2013 Yee Lian Chew. C. elegans – the roundworm. Small – 1 mm long in adults Anatomy has been fully mapped Highly stereotypical

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Nicholas lab: C. elegans research

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  1. Nicholas lab: C. elegans research Working with worms Postgraduate induction 2013 Yee Lian Chew

  2. C. elegans – the roundworm • Small – 1 mm long in adults • Anatomy has been fully mapped • Highly stereotypical • Reproduce sexually by mating with males and also by self-fertilisation (maintained as a haermaphrodite population) • Short-lived • Transparent

  3. What we do Hong Anna Aaron Hannah Sashi Duygu Matthew Yee Lian Slavica Priya Estelle Irene Mallory

  4. C. elegans – techniques we use • Generation of transgenic lines – biolistic transformation and microinjection • Molecular biology techniques including DNA/RNA work, qPCR, RNAi • Staining, western blot, mass spec (iTRAQ) • Microscopy – EM, confocal, fluorescence, dissecting scope • Dissections – germline staining • And of course, lots of fun with worms

  5. Worms are transparent – and yes, they have brains They have 302 neurons

  6. Co-localisation studies using transgenic C. elegans pdpy-7::HIS-24::mCherry pctbp-1::ctbp-1::gfp Merge Matthew Hoe

  7. Any questions? • Contact the boss: hannah.nicholas@sydney.edu.au

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