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Ribosome Structure/Function: The 50S subunit is a ribozyme!

Ribosome Structure/Function: The 50S subunit is a ribozyme!. Biochemistry 655 2 December 2009. A storied history. 1979 Ada Yonath decides to crystallize ribosomes - no pure ribosomes 1981 Ada Yonath produces the first crystals - no diffraction

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Ribosome Structure/Function: The 50S subunit is a ribozyme!

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  1. Ribosome Structure/Function:The 50S subunit is a ribozyme! • Biochemistry 655 • 2 December 2009

  2. A storied history • 1979 Ada Yonath decides to crystallize ribosomes - no pure ribosomes • 1981 Ada Yonath produces the first crystals - no diffraction • 1984 Ada Yonath studies cryoprotection - first diffraction • 1987 Ada Yonath collaborates on use of undecaGold for phasing • 1990-1996 Ada Yonath maintains labs at all synchrotrons • 1996 Ada Yonath collaborates with Marin Van Heel, Steitz and Moore collaborate with Joachim Franck • 1998 Steitz and Moore produce 9 Å electron density • 2000 Steitz and Moore produce 2.4 Å map of 50S • 2009 Yonath, Steitz, Ramakrishnan win the Nobel Prize in Chemistry

  3. Goals • Phase determination: the role of cryoEM • Noller’s Experiments; the dispensibility of protein • RNA tertiary structure • The Yarus inhibitor: CCdApPuromycin • The Yarus aptamers • The meek role of proteins • A1 target for macrolide antibiotics: blocking the “teflon tunnel”

  4. Nary a polypeptide near the active site

  5. Domain V

  6. Yarus: RNA aptomer selected for CCdAP-Puromycin affinity The selected eight-base sequence, AUAACAGG, is essentially completely conserved (>99%) among the known 23S rRNA sequences of eubacteria and chloroplasts and very highly conserved among all other phylogenetic domains (Gutell et al., 1993). 23S rRNA Similarity from Selection for Peptidyl Transferase Mimicry† Welch, Majerfeld, & Yarus* Biochem. 1997, 36, 6614-6623

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