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Rodent Associated Bartonella in Saskatchewan

Rodent Associated Bartonella in Saskatchewan. Claire Jardine ADED Rounds Nov. 26, 2003. Outline. Introduction Objectives Methods Results to date Plans for next year Summary . Bartonella. Gram-negative bacteria Parasitize mammalian RBC Vector-borne. Bartonella. Prior to 1992:

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Rodent Associated Bartonella in Saskatchewan

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  1. Rodent Associated Bartonella in Saskatchewan Claire Jardine ADED RoundsNov. 26, 2003

  2. Outline • Introduction • Objectives • Methods • Results to date • Plans for next year • Summary

  3. Bartonella • Gram-negative bacteria • Parasitize mammalian RBC • Vector-borne

  4. Bartonella • Prior to 1992: • Carrion’s disease – B. bacilliformis • Trench fever – B. quintana “medical curiosities” “bacteria from the past”

  5. After 1992: Urban trench fever Cat scratch disease Bacillary angiomatosis Parinaud’s oculoglandular syndrome Peliosis hepatis Uveitis Encephalitis Endocarditis Febrile illness Neuroretinitis Myocarditis Bartonella

  6. Bartonella

  7. Objectives • Determine if Bartonella occurs in selected small mammals in central Saskatchewan • Determine which isolates of Bartonella are infecting small mammals in central Saskatchewan

  8. Study Areas • Ten sites within 50 km radius of Saskatoon

  9. Culture

  10. 500 bp Samples 2-9 N P PCR

  11. Phylogeny

  12. Plan • Complete PCR, sequencing work • Develop phylogeny for all isolates

  13. Objective • Investigate the ecology of Bartonella infections in RGS

  14. Methods • Each month at 5 sites • Trap • Collect blood • Mark • Release

  15. Results

  16. Plans • Demographics • Follow individual animals • Relate Bartonella prevalence to flea prevalence and load • Determine the effect of experimentally reducing flea loads on Bartonella

  17. Summary • Bartonella occurs at high prevalence levels in small mammals in Saskatchewan • New strains associated with Richardson’s ground squirrels • Plan • Ecology • Fleas

  18. “The overall host-species diversity of Bartonella attests to the ability of members of this genus to adapt to a wide range of vertebrate hosts. This observation suggests that various members of the genus, not currently associated with human disease, are likely to continue to evolve to be sources of zoonotic and perhaps emergent human disease.” Karem et al. 2000

  19. Funding/Support MRC, WHF, MSFHR CDC, Fort Collins National Microbiology Lab, Winnipeg CCWHC/WCVM CWS Committee Dr. Leighton Dr. Wobeser Dr. Chirino Dr. Artsob Dr. Bollinger Dr. Jackson Acknowledgements Special Thanks: Dr. Kosoy from the CDC, Dr. Appleyard and the PCR lab, Dr. Chirino’s lab, and D. McColl from Health Canada

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