1 / 16

1 Department of Analytical and Food Chemistry 2 Department of Ecology and Animal Biology

Variation in the concentration of polycyclic aromatic hydrocarbons (PAHs) in mussels ( Mytilus galloprovincialis ) at the galician coast after the prestige oil spill. Ó. NIETO 1 , J. ABOIGOR 1 , L. SACO-ÁLVAREZ 2 and R. BEIRAS 2. 1 Department of Analytical and Food Chemistry

armen
Download Presentation

1 Department of Analytical and Food Chemistry 2 Department of Ecology and Animal Biology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Variation in the concentration of polycyclic aromatic hydrocarbons (PAHs) in mussels (Mytilus galloprovincialis) at the galician coast after the prestige oil spill. Ó. NIETO1, J. ABOIGOR1, L. SACO-ÁLVAREZ2 and R. BEIRAS2. 1 Department of Analytical and Food Chemistry 2 Department of Ecology and Animal Biology Faculty of Marine Sciences University of Vigo E-36310 Vigo, Spain

  2. www.lavozdegalicia.es LOS HÉROES DEL 13-N. La Voz de Galicia. Jan. 2003. p 12

  3. M1 Sampling stations M1: Furnas 42º 38’ 38” N, 9º 02’ 15” W

  4. Sampling stations M2: Vilar 42º 33’ 05” N, 9º 01’ 40” W M2 M2

  5. M3 M3 Sampling stations M3: Aguiño 42º 30’ 59” N, 9º 01’ 00” W

  6. Sampling dates

  7. Condition index Condition index (g m-1 d.w.) M1 M2 M3

  8. Sample preparation • The mussels collected are of 40 to 60 mm length • The soft tissue is taken out from the shell and frozen at -80ºC • Freeze-dry, trituration • Soxhlet extraction: • solvent n-Hexane : Acetone • 4 cycles per hour • Solid Phase extraction: • adsorbent: Florisil 10% deactivated • elution with n-Hexane. • Solvent evaporation and adition of 1 ml acetonitrile

  9. Determination: HPLC with fluorescence detection • Chromatographic system model alliance 2690 (Waters) • Fluorimetric detector model 474 (Waters) • Software Millenium 32 (Waters) • Column Vydac 201TP54, 4.6 x 250 mm, 5µm particle size, Grace Vydac. • Injection volume: 20 µL • Gradient elution with water:methanol • Column temperature: 23,5ºC • Detection by programming the excitation and emission wavelengths

  10. Sum of PAHs M1 M2 M3 date

  11. Percentage of parent PAHs November 2002 – April 2003 M3 M1 M2 June - December 2003 M3 M1 M2

  12. Parent PAHs ratio ΣLPAH = Nap + Ace + Flu + Phe + Ant ΣHPAH = Fla + Pyr + BaA + Chr + BbF + BkF + BeP + BaP + BghiP + DahA + Ipy

  13. Sum of 6 PAHs M1 M3 M2 date [BkF] + [BaA] + [BbF] + [BaP] + [DahA] + [Ipy] < 200 ng/g (d.w.)

  14. Ct = C0 exp(-rt) Depuration rate, r (days-1)

  15. Conclusions • Overstated concentrations of PAH are found in the mussel samples during the following months after the oil spillage • From April-June 2003, these concentrations are similar to those found in a non polluted area. • Similar percentages of parent PAHs, with a higher proportion of HPAH with respect to LPAH are found from November 2002 to April 2003. • The decrease in the PAH concentrations in the mussel samples can be adjusted to the following equation: • Ct = C0 exp(-rt) • Similar values of r are found for the studied hydrocarbons. • The results suggest two mechanisms for depuration of PAHs in mussels.

  16. Acknowledgements • MCyT (project VEM 2003-20068-C05-02) for financial support • Dr. Emilio Fernández Suárez and the rest of LEM research group for material support and collaboration in sampling campaings Thank you very much for your attention

More Related