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Bacterium-induced basal resistance inhibits viral infection in tobacco plants

Bacterium-induced basal resistance inhibits viral infection in tobacco plants. László ZSIROS 1,2 , Ágnes SZATMÁRI 1 , László PALKOVICS 2 , Zoltán KLEMENT 1 Zoltán BOZSÓ 1 1 Plant Protection Institute of the HAS 2 Corvinus University of Budapest Dept. of Plant Pathology.

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Bacterium-induced basal resistance inhibits viral infection in tobacco plants

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  1. Bacterium-induced basal resistance inhibits viral infection in tobacco plants László ZSIROS1,2, Ágnes SZATMÁRI1, László PALKOVICS2, Zoltán KLEMENT1Zoltán BOZSÓ1 1Plant Protection Institute of the HAS 2 Corvinus University of Budapest Dept. of Plant Pathology

  2. Hypersensitive response (HR) Basal resistance (BR) Early basal resistance (EBR) Late basal resistance (LBR) Main types of resistance in plants Microorganism-induced Preformed

  3. Comparison of basal and hypersensitive defense

  4. Flagellin, LPS Avr proteins Basal resistance Hypersensitive response Plant cell Induction of basal resistance (BR) and hypersensitive response (HR) Intercellular space

  5. Tobacco mosaic virus (TMV) • Resistance in tobacco • HR – 78 hours • N gene • Plant-TMV interaction • incompatible (HR) • compatible (mosaic leaves)

  6. Changes at cell level during basal resistance (BR) • change in pH level • cell wall fortification • papilla formation • active oxygen forms (eg. hydrogen peroxide) • bacterium envelopment

  7. Aims of research • Does a bacterial infection impede the symptoms/HR of a viral superinfection? • Is symptom/HR inhibition attached to bated virus replication? • How do some defense-related genes change in their transcription activity?

  8. Materials and methods • Plants • N. tabacum L. ‘Samsun’ nn (compatible) • N. tabacum L. ‘Xanthi’ NN (incompatible) • Bacterial inoculation • Pseudomonas syringae pv. syringae hrcC mutant • Viral inoculation • TMV • Virus quantity and gene activity assay • Real-Time PCR

  9. Bacterial inoculation Water infiltration (mock) Viral inoculation 6 hours TMV Inoculation method to assay early basal resistance (EBR)

  10. HR-inhibition assay (‘Xanthi’ NN plant, incompatible tobacco-TMV interaction) Bacterium (hrcC) + TMV Water + TMV Positive control (TMV only)

  11. HR-inhibition assay(‘Xanthi’ NN plant, incombatible tobacco – TMV interaction) Average results of repeated treatments

  12. Inhibition of virus replication(‘Xanthi’ NN plant, incombatible tobacco – TMV interaction) hrcC = Pseudomonas syringae pv. syringae hrcC mutáns Representative results of repeated treatments

  13. Defense-related genes(‘Xanthi’ NN plant, incompatible tobacco – TMV interaction) • Epoxide hydrolase – epoxide neutralization • Ortho-methyl trasferase – cell wall synthesis • Superoxide dismutase – superoxide degradation

  14. Defense-related genes 1(‘Xanthi’ NN plant, incompatible tobacco – TMV interaction) hrcC = Pseudomonas syringae pv. syringae hrcC mutant Representative results of repeated treatments

  15. Defense-related genes 2(‘Xanthi’ NN plant, incompatible tobacco – TMV interaction) hrcC = Pseudomonas syringae pv. syringae hrcC mutant Representative results of repeated treatments

  16. Defense-related genes 3(‘Xanthi’ NN plant, incompatible tobacco – TMV interaction) hrcC = Pseudomonas syringae pv. syringae hrcC mutant Representative results of repeated treatments

  17. Symptom inhibition of EBR(‘Samsun’ nn plant, compatible tobacco – TMV interaction) Water + TMV Bacterium + TMV TMV

  18. Inhibition of virus replication(‘Samsun’ nn plant, compatible tobacco – TMV interaction) hrcC = Pseudomonas syringae pv. syringae hrcC mutant Representative results of repeated treatments

  19. Summary • Related to EBR, there was inhibition observed in • mosaic leaf symptom development; • virus-induced HR development; • the rate of virus replication; • change in activity of some defense-related genes

  20. Conclusions • Possible reasons for HR or mosaic symptom inhibition during basal resistance: • blocking of virus translocation in plant • inhibition of virus replication (RNA silencing? priming?) • non-specific defense • hydrogen peroxide  symptoms

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