1 / 3

3500x

Supplementary Figure 1. A. TiO 2. 3500x. 8900x. B. Carbon black. 5600x. 14 000x. C. SWCNT. 1500x. 5000x. Supplementary Figure 2. P-2 κ B α immunostaining of WT and p53 KO macrophages. A. DMEM. SWCNT. p53 KO. WT. WT. p53 KO. Immunostaining quantification. B.

arnav
Download Presentation

3500x

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Supplementary Figure 1 A TiO2 3500x 8900x B Carbon black 5600x 14 000x C SWCNT 1500x 5000x

  2. Supplementary Figure 2 P-2κBαimmunostaining of WT and p53 KO macrophages A DMEM SWCNT p53 KO WT WT p53 KO Immunostaining quantification B Percentage of positively stained cells (%) P-IκBα DAPI Merge 2µg/cm2

  3. Supplementary figure legends SuppFig 1.Transmission electron microscopy images of RAW 264.7 cells incubated with 10 µg/ml(2 µg/cm2) of manufactured nanomaterials (MNMs) for 6 hr. (A) RAW 264.7 cell with TiO2 A10 MNMs within a vesicle (B) CB P60 MNMs within the cytosolic compartment of a RAW cell. (C) RAW macrophages with SWCNT at the membrane surface. Supp Fig 2.Consequence of p53 activation: inflammatory response in primary wild-type (WT) and p53 knock-out (KO) macrophages. (A, B) The cells were exposed to 2 µg/cm2 of TiO2 (A10), CB (FW2) and SWCNT MNMs for 30 min. The data show the percentages of phosphorylated IκBα (P- IκBα) positively stained macrophages (mean ± SEM) (n=3). * significant difference from WT control (p < 0.05). # significant difference between WT and p53 KO macrophages (p < 0.05).

More Related