Uses and Advantages of glycan array for the researchers

1. Uses and Advantages of glycan array for the researchers Glycan microarray is the set of immobilized glycans that is printed on the multi-well plated. It follows the event of successful DNA and recombinant protein microarray since the 20th century. With the glycan array technology, it is possible for analyzing the glycan-binding proteins prevalent in immunity, pathogens, and mammals that have aided research for cancer biomarkers, medical diagnostics, and vaccine production. When you are choosing the right glycan array, it will offer the most convenient tool to find the carbohydrate-binding characteristic of proteins, bacteria, antibodies, cell culture. Choosing the right manufacturer will also help you to have the custom glycan array based on the demands of the large library of glycans. Are you now looking for more information on the glycan array? Keep reading, and you will discover more about it. Know what Glycan array is Glycon array is the chemical product composed of different oligosaccharides and polysaccharides immobilized on the solid support in a spatially defined arrangement for some purposes. The technology will offer several means of study where you can know more about the interaction between the glycan and protein in the natural and chemical environment. Both the natural and synthetic and synthetic glycan can be incubated with the binding along with the protein like cell surface receptors, lectins, or possibly the whole organism like a virus. The

2. binding will also be quantified with the help of the fluorescence-based methods used for detections. Applications of the glycan array With the glycan array technology, it has still been applied to the study of certain specific host- pathogen interactions between the compounds. In the olden days, the glycan arrays were proven to be highly useful for determining the species of the hemagglutinin of the influenza A virus that will bind the host and distinguishing across several strains of flues that will also include the avian from the mammalian. It is the instance, where you can find the CFG array and the customized array. The cross-platform benchmarks will lead to the highlights and effects of the glycan presentation and scraping on the binding factors. When it comes to the glycan array, it is possibly combined with several other techniques like surface Plasmon resonance or SPR. It helps with refining the characteristic of glycan-binding for several purposes. For instance, the combination will help for demonstrating the calcium-based on heparin-binding of Annexin A1 that will involve these factors in several biological processes such as apoptosis, membrane trafficking, and inflammation.

3. Know how it works Immobilization: It is printed glycans, and it is also non-covalently immobilized to individuals. It also utilizes closely structured that reduces to the end of the glycans, typically aldehydes, chemistry, and ketones. For instance, the hydrazide functionalized slides will use some chemical reaction to immobilize the glycans. Protein building: It is the naturally isolated or chemo enzymatically synthesized glycon0-finding protein that is applied at the slides. Fluorescence labeling: This fluorescent label will be directly applied to the biotinylated proteins, or it will be attached to the secondary antibody of the compound. Detection: The array will be screened with the help of the fluorescence detector. Advantages of glycan array for the researchers Making use of the advancement in the microarray technology that is developed for the antibody array, the companies are now helping to develop the fine glycan array for screening protein- carbohydrate interactions. This array will help the researchers in several ways like, Profile the informatory immune response Profile substrate specificity of the enzymes like glycosidases, glycosyltransferases, and several others Finding whether the target proteins are carbohydrate-binding proteins Identifying the binding partners in the biological samples The glycans are spotted on the array, and this will help in capturing the glycan-binding proteins. For instance, the influenza virus will bind to varieties of silicides with the serotype-specific pattern. When looking for some other examples, Galectins are involved in apoptosis cell adhesion and T-cell activation suppression and functions by binding the beta galactosides. Glycan synthesis Generally, the glycan synthesis will be done through two methods. Label based method Sandwich based method Label based method

4. In this method, the proteins or any other antibodies in the sample are biotins labeled, and it will also allow the direct detection on the array through the equivalent compound dye-conjugated biotin-streptavidin complex. A complete protocol and the primary things needed for the procedure should be noted even before starting with the process. Sandwich based method This method is used for antibody detection of target proteins captured on the particular array. The user should usually have to supply the labeled reporter antibodies specifically for the protein-based on their interest. For example, you can consider an antibody cocktail. Specific antibody concentrations and conditions will usually require determining the needs of the end- user. Final thoughts Have you now got an overall idea for the glycan? So, you need to consider the based aspects for enjoying the benefits of it completely. To know more about: www.arrayglycan.com