Essential Lab Safety Training Guide by Michael Coleman at CAPS

1. CAPS’General Safety Training Presented by Michael Coleman – CAPS Safety Coordinator

2. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

3. General Lab Safety • Safety Don’ts • Do anything that you are not sure that you can do without hurting yourself or someone else • Wear open-toed shoes • Bring food into CAPS labs • Safety Do’s • Ask questions • Wear Personal Protective Equipment (PPE) • Inform Safety Coordinator of any unsafe working conditions • Report accidents immediately

4. General Lab Safety

5. Emergency Action Planning

6. General Lab Safety

7. General Lab Safety • Report any unusual leaks, smells or sounds • Be aware of rotating machinery • Report any unsanitary conditions (insects, mold, etc…) • Limit your exposure to hazardous materials

8. General Lab Safety Personal Protective Equipment (PPE) • PPE must be worn if engineering and administrative controls do not eliminate the hazard • Protective Clothing • Long sleeve pants/shirts • Closed shoes • Sturdy work shoe (no sandals, no open heels) • Eyewear • Safety glasses, goggles, or face shields (can be provided by CAPS) • Gloves • Leather, cryogenic, chemically resistive, etc. (can be provided by CAPS) • Hearing protection • Ear plugs/muffs (can be provided by CAPS)

9. General Lab Safety Engineering Controls • Fume Hoods • NEMA Enclosures Administrative Controls • Safe operating procedures

10. General Lab Safety Housekeeping • Keep walkways free of clutter • Clean up spills and leaks promptly • Dispose of waste materials properly • Store tools and equipment properly • Make sure work areas have adequate lighting • Report inadequately lit areas to CAPS Safety Coordinator • All laboratory personnel are responsible for maintaining a safe and compliant workspace

11. General Lab Safety Housekeeping

12. General Lab Safety Hazard Communication • Labeling & Marking • Blue: Health • Red: Flammability • Yellow: Reactivity • White: Special Instructions • 0 – Lowest Hazard • 4 – Highest Hazard • This is the old style, but you may find chemicals marked this way

13. General Lab Safety Hazard Communication • Laboratory Postings • Be familiar with the information on this sheet for your laboratory • If inaccuracies exist, please contact your PI, CAPS Safety Coordinator, or EH&S so that it can be updated with the most current information

14. General Lab Safety Hazard Communication • Globally Harmonized Systems • Current standard for hazard communications in industry • Link available at CAPS’ safety homepage • This is the new style • Numbering for hazards goes from low to high, with low numbers being most toxic, and high numbers being less toxic (OPPOSITE of TRADITIONAL MARKINGS) • Both old an new styles may be found in your work space. Be aware of what you are reading

15. General Lab Safety Materials Handling & Storage • Do NOT attempt to move something that is awkward or heavy by yourself • Facilities personnel have access to: manpower, forklifts, pallet jacks, various carts, and other equipment that you can use to move heavy or awkward loads • Use of forklifts and cranes requires specialized training • If you require assistance moving something at CAPS, contact either: • CAPS Safety Coordinator • CAPS Facilities Coordinator

16. General Lab Safety Reaching High Places • Use a Ladder • Do not stand above the second rung • Stand on the “step-side” of the ladder • Do not Overreach • Do not stand on: • Stools • Chairs • Things other than ladders

17. General Lab Safety • PI’s and Lab Supervisors are responsible for: • developing lab-specific safety policies • ensuring that appropriate PPE is available to all CAPS’ personnel under their supervision • educating CAPS’ personnel under their supervision about the specific hazards associated with their research • encouraging CAPS’ personnel under their supervision to comply with CAPS’ safety policies

18. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

19. Hazardous Waste Awareness • Required by FSU’s Department of Environmental Health & Safety for access into any university laboratory • Link on CAPS’ safety homepage

20. Hazardous Waste Awareness

21. Hazardous Waste Awareness • Everyone who is granted access to a lab at CAPS must: • Take the EH&S Hazardous Waste Awareness training • Understand that all chemicals must be labeled • Provide the CAPS Safety Coordinator with any MSDS sheets for chemicals brought into the laboratory

22. Hazardous Waste Awareness • No hazardous Waste is to be disposed of • Down a drain • In the trash • In recycle bins • The examples above are a violations of state and federal laws • Contact the CAPS Safety coordinator or EH&S for coordination of hazardous waste removal from your lab

23. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

24. Electrical Safety

25. Electrical Safety • Everyone who is granted access to a lab at CAPS must: • recognize lock out/tag out devices and know what to do when they are encountered • understand the hazards associated with electrical equipment • be aware of required PPE for electrical safety

26. Electrical Safety Lock Out/Tag Out • Lockout/tag out is a procedure used to isolate potential sources of energy during repair or maintenance of equipment • Only the person who installed the lock may remove it • Never • attempt to operate locked/tagged out equipment • tamper with locks or tags that you did not install

27. Electrical Safety Primary hazards of electricity: • Electric Shock • Electric Burns • Arc blast Secondary Electricity Hazards • Explosions, • Electrical Fires, and • Secondary Injuries

28. PPE: Rubber gloves, sleeves Eye protection Non-flammable clothing Electrically insulated boots PPE shall NEVER be used as a substitute for de-energizing equipment or circuits Electrical Safety

29. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

30. Cryogen Safety

31. Cryogen Safety • Everyone who is granted access to a lab at CAPS must: • understand the hazards associated with cryogenic equipment • be aware of required PPE for cryogen safety

32. Cryogen Safety Hazards • Asphyxiation • When released in poorly ventilated areas • Cold Injury • Over Pressurization • Fire • with cryogens that have a lower boiling point than oxygen (90 K)

33. Cryogen Safety PPE • Goggles • or combination face shield and safety glasses • Loose cryogen gloves • Long sleeved shirts/lab coat • Close-toed shoes • Long Pants • Remove metal jewelry

34. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

35. Compressed Gas Safety

36. Compressed Gas Safety • Everyone who is granted access to a lab at CAPS must: • understand the hazards associated with compressed gas equipment • be aware of required PPE for cryogen safety

37. Compressed Gas Safety • Hazards • Explosion • Tank rupture causing uncontrolled gas release • Projectile • Valve shear causing directed gas release • Crushing • From weight of cylinder on appendages • Asphyxiation • When released in poorly ventilated areas • Fire Hazard • Not necessarily; refer to MSDS

38. Compressed Gas Safety • PPE • Safety Glasses, Face Shield or Goggles

39. TABLE of CONTENTS • General Lab Safety • Hazardous Waste Awareness • Electrical Safety • Cryogen Safety • Compressed Gas Safety • Emergency Action Planning

40. Emergency Action Planning

41. Emergency Action Planning • Emergency Contacts • Your PI/laboratory supervisor • 911

42. Emergency Action Planning • Know what to do in case of an emergency situation • Review the emergency action plan for your laboratory • Know the emergency equipment and procedures prior to beginning an experiment • Inform coworkers of the hazards of the experiment being performed • Always wear personal protective equipment

43. Emergency Action Planning Laboratory Emergency Equipment CAPS Maintains • 11 First Aid Kits • 9 Emergency shunt trip buttons • 8 Fire alarm pull stations • 18 Fire extinguishers • 2 Safety showers & eye wash stations • 1 Automatic Electronic Defibrillator (AED)

44. Emergency Action Planning • First Aid Kits • Emergency shunt trip buttons • Fire alarm pull stations • Fire extinguishers • Safety showers & eye wash stations • AED Laboratory Emergency Equipment 1 1 1 1 1 1 1 1 1 1 1

45. Emergency Action Planning • First Aid Kits • Report missing materials using inventory sheet

46. Emergency Action Planning • First Aid Kits • Emergency shunt trip buttons • Fire alarm pull stations • Fire extinguishers • Safety showers & eye wash stations • AED Laboratory Emergency Equipment

47. Emergency Action Planning • DO NOT enter the breaker cabinets in CAPS’ lab spaces for any reason • Coordinate with CAPS’ Facilities Coordinator to ensure that your experiment is properly connected to the emergency shunt trip system • In an emergency situation, press an emergency shunt trip button to remove power from your experiment in CAPS labs • Lighting and non-experimental circuits are not affected by engagement of the emergency shunt trip system

48. Emergency Action Planning • First Aid Kits • Emergency shunt trip buttons • Fire alarm pull stations • Fire extinguishers • Safety showers & eye wash stations • AED Laboratory Emergency Equipment 1 1 1 1 1 1 1 1

49. Emergency Action Planning • First Aid Kits • Emergency shunt trip buttons • Fire alarm pull stations • Fire extinguishers • Safety showers & eye wash stations • AED Laboratory Emergency Equipment 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

50. In Case of Fire Call 911 or campus police at 644-1234 immediately If fire Alarm has not sounded pull manual pull station If it can be done safely, shut off power to burning equipment or area If you are adequately trained Help injured personnel if safe to do so (DON’T BECOME A VICTIM) Extinguish with appropriate fire extinguisher If the fire is small fire, and you are qualified: extinguish with appropriate fire extinguisher Class C (dry chemical), CO2, or Halon. (Halon and CO2 are better for electrical fires) Never use water or foam extinguisher on electrical fires. Emergency Action Planning