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Similarity. Strain. RFLP type. 100. 70. 60. 65. 75. 80. 85. 90. 95. 50. 55. CCBAU 83850. CCBAU 83855. CCBAU 83879. E . meliloti USDA 1002 T. CCBAU 83574. CCBAU 83595. CCBAU 83875. CCBAU 83866. CCBAU 83893. CCBAU 83881. CCBAU 83571. CCBAU 83852. CCBAU 83572.
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Similarity Strain RFLP type 100 70 60 65 75 80 85 90 95 50 55 CCBAU 83850 CCBAU 83855 CCBAU 83879 E. meliloti USDA 1002T CCBAU 83574 CCBAU 83595 CCBAU 83875 CCBAU 83866 CCBAU 83893 CCBAU 83881 CCBAU 83571 CCBAU 83852 CCBAU 83572 CCBAU 83576 CCBAU 83575 CCBAU 83891 CCBAU 83578 CCBAU 83889 CCBAU 83856 CCBAU 83896 CCBAU 83577 CCBAU 83890 CCBAU 83895 CCBAU 83886 CCBAU 83860 CCBAU 83898 CCBAU 83862 1 CCBAU 83865 CCBAU 83849 CCBAU 83590 CCBAU 83851 CCBAU 83848 CCBAU 83874 CCBAU 83877 CCBAU 83847 CCBAU 83873 CCBAU 83867 CCBAU 83857 CCBAU 83883 CCBAU 83859 CCBAU 83858 CCBAU 83854 CCBAU 83892 CCBAU 83887 CCBAU 83884 CCBAU 83900 CCBAU 83864 CCBAU 83853 CCBAU 83899 CCBAU 83897 CCBAU 83870 CCBAU 83876 CCBAU 83871 CCBAU 83868 CCBAU 83573 CCBAU 83603 CCBAU 83589 CCBAU 83880 CCBAU 83894 CCBAU 83579 3 E.medicae USDA 1037T CCBAU 83882 CCBAU 83888 2 CCBAU 83600 CCBAU 83596 CCBAU 83885 Supplementary Fig. S1. Dendrogram based on PCR-RFLP patterns of nodC showing the relationships of the Trigonella arcuata rhizobia. nodC types are based upon the combined restriction patterns with endonuclease MspI, HaeIII and RsaI.
CCBAU 83884 (EU581817) CCBAU 83881 (EU581816) Trigonalla arcuata rhizobia (nodC type 1) 93 93 CCBAU 83856 (EU379969) CCBAU 83848 (EU581815) 81 81 CCBAU 83887 (EU379970) E. meliloti ATCC 9930T (M55229) 53 53 E. medicae A321T (DQ45093) E. kostiense HAMBI 1489T (Z95216) E. saheli ORS 609T (Z95221) 69 69 E. arboris HAMBI 1552T (Z95214) 57 57 E. terangae HAMBI 1552T (Z95214) R. etli CFN 42T (M15942) 84 84 M. mediterraneum Rch-984 (AJ457916) 64 64 R. gallicum R602spT (AF218126) 55 55 R. hainanense CCBAU 57015T (AY934876) 90 90 M. huakuii CCBAU 25056 (EF153402) E. fredii USDA 191 (Z95225) E. americanum CFNEI 156T (AF506514) B. elkanii USDA 76T (AB094963) B. japonicum IAM 12608 (AB079619) 96 96 B. yuanmingense CCBAU 10071T (AY599090) 0.02 Supplementary Fig. S2. Phylogenetic tree based on nifH sequences constructed with the neighbor-joining method and the Kimura 2-parameter model, showing relationships of Trigonalla arcuata rhozobia (in boldface) with other rhizobia in Ensifer (E.), Rhizobium (R.), Mesorhizobium (M.) and Bradyrhizobium (B.). Bootstrap values greater than 50% are indicated in the main nodes. The scale bar represents 2% nucleotide substitution.