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Ichthyophthirius multifiliis. Presentation by: Lauren Hannan and Chloe Jensen. Kingdom: Protista Phylum: Ciliophora Class: Oligohymenophorea Order: Hymenostomatida Family: Ichthyophthiriidae Genus: Icthyophthirius Species: multifillis. Morphology.
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Ichthyophthiriusmultifiliis Presentation by: Lauren Hannan and Chloe Jensen
Kingdom: Protista Phylum: Ciliophora Class: Oligohymenophorea Order: Hymenostomatida Family: Ichthyophthiriidae Genus: Icthyophthirius Species: multifillis
Morphology • Adults are as large in diameter as 1 mm • Macronucleus is a large, horseshoe-shaped body that encircles the tiny micronucleus • 3 life stages: • Feeding stage: trophont/trophozoite • Reproductive stage: tomonts/tomites • Infective stage: trophozoites/tomites/theronts/swarmers
Geographic Distribution: It is wide spread in all freshwater fish but appears to be more common in aquarium fish, possibly due to the closer contact and stress involved with aquarium species. Definitive Hosts: All freshwater fish Intermediate Hosts: None
Life Cycle • Feeding stage: trophont/trophozoite • Attached to the epithelial cells and absorbs nutrients from the fish • Reproductive stage: tomonts/tomites • Quickly attaches to a substrate and encysts • Spends all of its energy rapidly multiplying • Infective stage: trophozoites/tomites/theronts/swarmers • Tomites have burst from cyst • Sprout cilia and immediate start looking for a host • Typically only have a few days to find a host or they perish
Sites of Infection Skin Eyes Gills
Pathogenosis • Greyish/white pustules • Round or oval in shape • Roughly the size of a grain of salt • Epidermal cells produce mucous for protection • Death if not treated
Behavioral Signs • Wasting • Hiding abnormally • Rubbing against items • On skin: • White pustules • On eyes: • Cloudy/milky • On gills • Breathing at surface • Rapid breathing • White pustules
Diagnoses Observation of fish Study of pustules/parasite under microscope
Treatment • In aquarium fish: • Very dilute concentrations • Formaldehyde • Malachite green • Methylene blue • Commercial preparations
Control Food with malachite green Purchase healthy fish Clean tank Isolate sick fish