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報告者 : 劉 銨 錤

γδ T cell homeostasis is established in competition with αβ T cells and NK cells Jena D. French, Christina L. Roark, Willi K. Born, and Rebecca L. O’Brien* PNAS I October I vol. 102 l no. 41 l 14741-14746. 報告者 : 劉 銨 錤. Introduction. αβ T cells. CD8 or CD4 (TCRcoreceptor).

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報告者 : 劉 銨 錤

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  1. γδ T cell homeostasis is established in competition with αβ T cells and NK cellsJena D. French, Christina L. Roark, Willi K. Born, and Rebecca L. O’Brien*PNAS I October I vol. 102 l no. 41 l 14741-14746 報告者: 劉 銨 錤

  2. Introduction αβ T cells CD8 or CD4 (TCRcoreceptor) CD3(TCR complex) T cell receptor (TCR)

  3. Introduction γδT cells CD3(TCR complex) T cell receptor (TCR)

  4. αβT cell

  5. Nature killer cell

  6. IL-7:pre-T cell生長因子 • IL-2 : T cell 生長因子，刺激T cell生長與分化 • IL-15 : 刺激 T cell增生與分化及NK cell活化 • IL-2 receptor 和IL-15 receptor的β chian相同

  7. Materials • Mice wild-type C57BL/6 基因剔除strain﹕ C57BL/6 TCRδ-/- C57BL/6 TCRβ-/- C57BL/6 TCRβ-/-/δ-/-

  8. Methods • 從活體中萃取純化出各種 T cellIs 1.nylonwool column 2.Magnetic-actived cell sorting(MACS) • Flow cytometry • Adoptive transfer

  9. 磁 鐵 磁 鐵 磁珠 專一性抗體 細胞

  10. Mechanismof Flow Cytometry

  11. recipients TCRβ-/-/δ-/- mice TCRβ-/-mice Both γδ T cell and αβT cell regulate γδ T cell homeotasis.

  12. recipients: TCRβ-/-/δ-/- mice 在維持γδ T cell homeotasis中, CD8+ αβ T cell 扮演的角色比CD4+αβT cell

  13. recipients: TCRβ-/-/δ-/- mice PBS IL-7 IL-15 recipients: TCRβ-/-/δ-/- mice αβT cell 藉由與 γδ T cell競爭IL-15, 來抑制 γδ T cell增生分化

  14. IL-7Rα IL-2Rβ

  15. recipients: TCRβ-/-/δ-/- mice NK cells 也會抑制 γδ T cell增生分化

  16. recipients: TCRβ-/-/δ-/-mice cotransfer mice received PBS or IL-15 recipients: TCRβ-/-/δ-/- mice αβT cell 會與 γδ T cell競爭IL-15, 來調節並維持γδ T cell homeotasis

  17. Conclusion • γδ T cell homeostasis is regulated not only γδ T cell themselves but also by αβ T cells and NK cells. • αβ T cells and NK cells regulate γδ T cell homeostasis by competing IL-15. • CD8+ αβ T cells are largely reponsible for the inhibitory effect αβ T cells on γδ T cell homeostasis.

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