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1. 4. mAU. 175. 2. 6. 5. 150. 3. 7. 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid. 125. 100. 75. 50. 25. 0. 0. 0.25. 0.5. 0.75. 1. 1.25. 1.5. 1.75. 2. min. mAU. 1. 175. 4. 150. 2.

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  1. 1 4 mAU 175 2 6 5 150 3 7 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid 125 100 75 50 25 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min mAU 1 175 4 150 2 6 125 5 7 100 3 3.5 um 75 50 25 LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18) Flow: 2.0 ml/min 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min mAU 1 4 175 2 6 150 5 3 7 5 um 125 100 75 50 25 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min Aumento della Risoluzione in funzione del Particle Size 1.8 um

  2. Piccola quantità di particelle più grandi Meno particelle più piccole

  3. 0 1 2 3 4 5 6 7 min Aumento della Risoluzione in funzione del Particle Size 5µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) 1.8µm: Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 Rs = +50% Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza

  4. 1 2 3 Rs(1,2) = 3.1 4 0.5 1 1.5 2 2.5 1100 HT + 1.8um RRHT : • Reduction of analysis time from 30 min to 2 min • Speed: 14x faster than 4.6 x 250mm, 5um • Time saving: 92% • Resolution: -35% AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100 1 2 3 4.6 x 250 mm, 5 mm 1 mL/min Rs(1,2) = 4.8 29.65 N=21848 4 N=22680 0 5 10 15 20 25 30 min 1 2 3 Rs(1,2) = 3.5 12.71 4.6 x 100 mm, 3.5 mm 1 mL/min 4 N=11691 1 0 5 10 15 20 25 30 min . 2 3 N=6568 Rs(1,2) = 3.3 4.6 x 30 mm, 1.8 mm 1 mL/min 4.15 4 N=6104 0 5 10 15 20 25 30 min N=6463 4.6 x 30 mm, 1.8 mm 2 mL/min 2.09 N=6460 0 5 10 15 20 25 30 min

  5. Ultra-fast analysis in 26 sec 4 peak width ~ 0.6 sec < 50 sec cycle time 7 analgesics separated in 21 sec for peaks 1,2,3 Total time Injection-to-Injection Higher flow rate: higher speed – same resolution – higher sensitivity/precision Sample: 7 Analgesics Column: Zorbax RRHT SB-C18, 1.8 um, 4.6 x 30 mm 2 ml/min – 90bar Rs2,1 = 2.2 Rs7,6 = 2.2 3 ml/min – 133bar 4 ml/min – 181bar Rs2,1 = 2.1 Rs7,6 = 2.2 2x faster than 2ml/min without loss in resolution! Speed

  6. HPLC, 40°C SB-C18 4.6 x 150mm, 5µm 1.20ml/min, 40°C Analysis Time = 11min min 0 2 4 6 8 10 RRHT SB-C18 2.1mm x 50mm 1.8µm 1.00ml/min, 40°C Analysis Time= 1.1min RRLC, 40°C 10x faster 0.2 0.4 0.6 0.8 1 min 0 RRHT SB-C18 2.1mm x 50mm 1.8µm 2.40ml/min, 95°C Analysis Time: 0.4min RRLC, 95°C 27x faster 0.2 0.4 min 0.6 0 Rapid Resolution HT Columns – Up to 20X Faster • Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over) • Compliant with strictest regulatory performance requirements >20X faster

  7. 4 5 0 1 2 3 4 5 6 7 min NON SOLO VELOCITA’ MA ANCHE EFFICIENZA 5µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) 1.8µm: Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 Rs = +50% Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza

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