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Flu + ve

Referred samples. Outbreak/ Surveillance Sample. Isolates /clinical samples. Record History on different forms`. Sample Collection. Aliquot & Storage. NPA Sample. NS & TS Samples. Sample considered - ve. Isolation. MDCK. Direct and Indirect Immunoflurescence Test. Flu + ve. EGG.

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Flu + ve

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  1. Referred samples Outbreak/ Surveillance Sample Isolates /clinical samples Record History on different forms` Sample Collection Aliquot & Storage NPA Sample NS & TS Samples Sample considered -ve Isolation MDCK Direct and Indirect Immunoflurescence Test Flu +ve EGG No Isolation 2nd Passage No Isolation Low titer Sample >1:8 Low titer Sample >1:8 Haemagglutination Test Test Results Verify Sample Titer Verify Sample Titer Diagnostic PCR for Low Titer Genetic Analysis HA +ve Haemagglutination Inhibition test

  2. Immune Sera Preparation Genetic Analysis Virus Preparation PCR Pre Serum Collection Immunization of Fowl Sequencing Post Serum Collecton Sequence Analysis Post Booster Serum Collection Collect Immune Serum

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