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CD4 GLP Protocols Clinical Standard Operating Procedures for CD4 Enumeration by Flow Cytometry Using the BD FACSCount S

Laboratory Safety. Biohazard SafetyGeneral PoliciesApprovalsWaste disposalAccident reporting and follow-upSafety committee. Specimen Processing. General PoliciesApprovalPeriodic reviewSpecimen CollectionPhlebotomyTrainingDocumentationSpecimen Handling and TransportSpecimen Requisition,

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CD4 GLP Protocols Clinical Standard Operating Procedures for CD4 Enumeration by Flow Cytometry Using the BD FACSCount S

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    1. CD4 GLP Protocols Clinical Standard Operating Procedures for CD4 Enumeration by Flow Cytometry Using the BD FACSCount System Burt Houtz, CLS Technical Adviser, Flow Cytometry Applications BD Biosciences This Get With The Flow seminar is presented by the Customer Education Services department.This Get With The Flow seminar is presented by the Customer Education Services department.

    2. Laboratory Safety Biohazard Safety General Policies Approvals Waste disposal Accident reporting and follow-up Safety committee

    3. Specimen Processing General Policies Approval Periodic review Specimen Collection Phlebotomy Training Documentation Specimen Handling and Transport Specimen Requisition, Receipt and Assessment Specimen Processing and Storage

    4. Specimen Identification Log Proper Identification Condition of the Specimen Date and Time Collection Receipt Source Lab location

    5. Immunofluorescent Labeling Preparing Controls Preparing Samples Vortexing Pipetting Incubation Times

    6. FACSCount Quality Control Startup Procedure Priming the System Entering Control and Reagent Information Running Controls

    7. FACSCount Quality Control Report Control Run Result Pass/Fail CD3 Range CD4 Range Process Control Reporting

    8. FACSCount Analysis of Specimens Startup Procedure Priming the System Entering Patient and Reagent Information Running Patient Samples Cleaning Procedure Shutdown Procedure

    9. Laboratory Reporting of CD4 Test Results Laboratory Worksheet Recording Patient Test Results

    10. FACSCount Maintenance Daily Cleaning Monthly Drain / Fill Cycle Annually System Fluid Filter Change

    11. FACSCount Maintenance Record Recording Data About Service Visits

    12. CD4 Enumeration Quality Assurance Quality Control Procedures Internal Controls Evaluate Patient Data T-Sum CD3 Consistency Check Positive Reagent Control Specimen Integrity Reagent Quality Control

    13. CD4 Enumeration Quality Assurance Reference Ranges Proficiency Testing Clerical Processing Quality Improvement Use of the Reagent Log

    14. Data Reporting of CD4 Test Results Supervisory Review Interpret in the Proper Context Provide Appropriate Information Provide Legible Results Retain Files for a Sufficient Length of Time Notify the Physician If Results Are Below a Critical Level Specify a Turnaround Time Control Patient Data

    15. Proficiency Testing Formal Programs CAP NEQAS Peer Review Processes Country-Based Organizations Specimen Concerns

    16. Proficiency Testing

    17. Clinical Training What is it? Why it is Important Know the Audience (if you can!) Lesson Plan Feedback

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