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Make it Rigid

Make it Rigid. Ca++. Application: Calcium sensing. Stark effect. Application: Membrane Voltage Sensing. TIRF Microscope. Setting up the TIRF microscope. Objective-TIRF. Prism-TIRF. Sensitivity of Fluorescence Microscope. Signal New concept: absorption cross-section

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Make it Rigid

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  1. Make it Rigid Ca++ Application: Calcium sensing

  2. Stark effect Application: Membrane Voltage Sensing

  3. TIRF Microscope

  4. Setting up the TIRF microscope Objective-TIRF Prism-TIRF

  5. Sensitivity of Fluorescence Microscope • Signal • New concept: absorption cross-section • Background due to excitation light: ~105 x (signal from one dye molecule) • Noise • Shot noise (unavoidable) • Thermal background noise • Read noise

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