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Principle of Fluorescence Light Microsocpy.

Principle of Fluorescence Light Microsocpy. The Confocal Fluorescence Microscope. Fluorescence Microscopy of Listeria Motility. Bacterium – green Actin - red. Julie Theriot Stanford University Medical School. Analysis of Mitosis in Drosophila Embryos: Immunofluorescence Microscopy.

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Principle of Fluorescence Light Microsocpy.

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  1. Principle of Fluorescence Light Microsocpy.

  2. The Confocal Fluorescence Microscope

  3. Fluorescence Microscopy of Listeria Motility Bacterium – green Actin - red Julie Theriot Stanford University Medical School

  4. Analysis of Mitosis in Drosophila Embryos: Immunofluorescence Microscopy.

  5. Fluorescence Analog Cytochemistry of Mitosis in the Drosophila Embryo. RITC-Tubulin (MTs) GFP-CenpA (Kinetochores) GFP-Tubulin (MTs) RFP-Histone (chromosomes)

  6. FRAP and FSM: Drosophila spindle MTs display poleward flux AND dynamic instability.

  7. Motility Assay of Purified Kinesin Motors Moving MTs Made of Fluorescent Tubulin.

  8. TIRF Microscopy Motility Assays.

  9. Optical Trapping and Motor Stepping

  10. Fluorescence Microscopy of the C.elegans Nervous System Expressing GFP from a Neuron-Specific Promoter.

  11. In vivo Time-lapse Fluorescence Motility Assays of IFT Kymography. (Orozco et al, 1999, Nature, 398, 674; Signor et al, 1999, JCB, 147:519)

  12. Immunofluorescence and Time-lapse Fluorescence of Kinesin-5 (Sharp et al, 1999, JCB, 144, 125; Cheerambathur 2008, JCB, 182, 429)

  13. Kinesin-5 Crosslinks localize to ipMT bundles (Sharp et al, 1999, JCB, 144, 125; Cheerambathur et al, 2008, JCB)

  14. FRAP Microscopy: Kinesin-5 and Tubulin display rapid dynamics KLP61F-GFP GFP-Tubulin t1/2 ~ 6s t1/2 ~ 5s

  15. Immunofluorescence Microscopy: Myosin-2 in Cytokinesis.

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