1 / 10

Jim Kolmer Maria Ordoñez USDA-ARS Cereal Disease Laboratory St. Paul MN

Genetic differentiation of molecular genotypes and virulence phenotypes of Puccinia triticina in Europe and world-wide. Jim Kolmer Maria Ordoñez USDA-ARS Cereal Disease Laboratory St. Paul MN. Thanks to Kun Xiao. European P. triticina isolates. Czech Rep. Slovakia - 20 Germany - 2

ayanna
Download Presentation

Jim Kolmer Maria Ordoñez USDA-ARS Cereal Disease Laboratory St. Paul MN

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Genetic differentiation of molecular genotypes and virulence phenotypes of Puccinia triticina in Europe and world-wide. Jim Kolmer Maria Ordoñez USDA-ARS Cereal Disease Laboratory St. Paul MN Thanks to Kun Xiao

  2. European P. triticina isolates Czech Rep. Slovakia - 20 Germany - 2 Spain - 3 France - 8 England - 35 Hungary - 6 Italy - 15 Romania - 2 Turkey - 39 Ukraine - 2 133 isolates - 122 after clone correction

  3. 23 SSR primer pairs (18 CDL-5 Fr) 77 SSR genotypes 20 Tc lines 89 virulence phenotypes

  4. SSR Genotype Grouping Instruct – Bayesian methodology (no assumption of HW equilibrium) K means clustering – maximize sum of distance2 between groups – minimize within groups Consensus tree – bootstrapping All methods indicate 8 SSR groups – identical grouping of isolates Most groups cosmopolitan (except EU7)

  5. EU SSR Groups RST RST = 0.50 EU2-EU7 ns EU2-EU8 ns EU7-EU8 ns All other pairs significantly different Stepwise mutation model – alleles close in mw more related Reflect long term evolutionary relationships? Homoplasy?

  6. EU SSR Groups FST FST = 0.32 All pairs significantly different Assumes infinite alleles model All alleles equally related Genetic drift is source of variation Detect recent immigrants better?

  7. EU SSR GroupsVirulence phenotypes PHI = 0.46 EU3-EU5 ns All other SSR groups significantly different for virulence SSR genotype – virulence phenotype correlation = 0.55

  8. World-wide P. triticina SSR groups North America South America Central Asia Middle East Europe New Zealand South Africa Durum isolates (EU, SA, ME, NA) Aegilops speltoides 587 isolates FST = 0.331 Some geographical relationship SA, NA vs. EU, ME, CA, NZ, SAF Except ME1 with NA and SA Durum groups distinct from common wheat groups

  9. World-wide P. triticina SSR groups RST = 0.561 Little geographic relationship SSR groups from different continents closely related many pairs ns different Two major groups from common wheat – distinct from durum groups

  10. Intercontinental migration ofP. triticina? RST grouping Highly related SSR groups have identical or similar virulence phenotypes MBDSS-MCDSS Mexico – early 1990s North America -1996 (NA3) South America- 1999 (SA3) France – early 2000s (EU8) Turkey- 2009 (EU8)

More Related