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Small RNA and Cyanobacteria

Small RNA and Cyanobacteria. Characteristics of Cyanobacteria. Aquatic Photosynthetic Usually unicellular. Atmosphere. Chloroplast. Importance of Cyanobacteria. What is sRNA?. Encoded in the intergenic regions. Why do we want to learn about sRNAs?.

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Small RNA and Cyanobacteria

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  1. Small RNA and Cyanobacteria

  2. Characteristics of Cyanobacteria • Aquatic • Photosynthetic • Usually unicellular

  3. Atmosphere Chloroplast Importance of Cyanobacteria

  4. What is sRNA? • Encoded in the intergenic regions

  5. Why do we want to learn about sRNAs? • To predict them in any genome of an organism • To experimentally analyze their function • To control gene expression

  6. Why have few sRNAs been found? • Very small (usually 40-300 nt) • Less sensitive to point mutations • Function at the 2º structure level • Do not encode protein sequence • No coding signals (start/stop codon)

  7. How Biolingua can search for sRNAs in cyanobacteria? • Compare the sequences of intergenic regions by BLAST • These results will include both an e-value and a percent identical score • Run the similar sequences from multiple organisms thru RNAz, which predicts if an RNA exists by its 2º structure

  8. To further verify an sRNA… • M-Fold: takes a potential RNA sequence and incorporates its structure • Experiments: test by northern blot analysis

  9. Acknowledgements: Jeff Elhai and Dr. You Chen

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