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ProVision 360

Commercialized Version of Explosives Detection Personnel Portal with IMS Detection. ProVision 360. Mass spectrometers at the Institute of Microbiology, Prague, Dec 1, 2006. Mass Spectrometer. Ionization. Detector. Normal pressure. Reduced pressure. Sample introduction. Mass Analyzer.

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ProVision 360

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  1. Commercialized Version of Explosives Detection Personnel Portal with IMS Detection ProVision 360

  2. Mass spectrometers at the Institute of Microbiology, Prague, Dec 1, 2006

  3. Mass Spectrometer Ionization Detector Normal pressure Reduced pressure Sample introduction Mass Analyzer Computer • Sensitivity • Specificity • Speed • Simplicity of data interpretation Well recognized unique attributesof Mass Spectrometry:

  4. Rozpouštědlo + + + + + + + + + Vyhřívaná kapilára ESI hrot (5 kV) Iontový svazek Vstupní otvor (nominálně 0-50 V) DESORPČNÍ TECHNIKY • Desorpce polem (Beckey) 1974 Desorpce plazmatem 252Cf (McFarlane) • Ionizace rychlými atomy (Barber) • Elektrosprej (Fenn)

  5. DESORPČNÍ TECHNIKY • Desorpce polem (Beckey) 1974 Desorpce plazmatem 252Cf (McFarlane) • Ionizace rychlými atomy (Barber) • Elektrosprej (Fenn) • Desorpce laserem za přítomnosti matrice (Tanaka, Karas, Hillenkamp)

  6. BLOOD ANALYSIS Mass calculated: 616.1767 Mass measured: 616.1761 C34H32N4O4Fe

  7. ORBITRAP: Retainment of the rings • Frequencies are determined using a Fourier Transform • For high sensitivity AND resolution needs ultra-high vacuum

  8. SORI of m/z 740.4703 RESULT: DIFFERENT AA RESIDUES ARE PRESENT!!!

  9. 9 8 7 6 5 4 -y LGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLF S b- 26 27 28 29 30 31 32 33 34 35 36 y6 y5 y7 y9 y4 y8 400 800 1200 1600 2000 2400 b34 b35 b31 b32 b36 b33 M+H b30 * b28 * * b29 b27 * b26 * * * * 2800 3200 3600 4000 m/z

  10. ~ ~ 36 35 36 35 34 33 32 31 30 29 28 27 26 25 24 23 13 7 4 3 - z LGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLF S c - 5 6 7 8 9 10 11 12 13 14 15 16 17 19 20 23 24 25 26 27 28 29 30 31 32 33 34 35 36 c16 c13 c17 c20z21 z3 z20 c9 c15 c10 c11 c6 c14 c5 c19 c8 c12 z4 c7 z7 z13 400 800 1200 1600 2000 2400 M+H z35 z25 c36 c34 z24 z36 c33 z32c32 z23 c35 z28 c29 z26 c30 c28 z27 z31 z22 c24 c27 c26 z29 c23 c31 z30 2800 3200 3600 4000 m/z

  11. IRMPD / ECD: Carbonic Anhydrase (29 kDa) Top Down

  12. MASS MICROSCOPY MALDI-LIFT-TOFTOF molecular images reconstructed from the data obtained by scanning two adjacent rat brain sections chloroform treated: R. Lemaire et al., Anal. Chem. 2006, eprint ahead

  13. J. Mass Spectrom. 2006; 41: 705–716

  14. TRENDY HMOTNOSTNÍ SPEKTROMETRIE - 2006 • Miniaturizace • Příprava vzorku – eliminace práce (DESI a jet-assisted DESI) • Nové analyzátory (orbitrap) / iontová optika (zvýšení citlivosti a přesnosti) • Mass microscopy: tissue imaging • Vysoce-rozlišující metody: elementární složení a moderní disociační techniky

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