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A. B. EELCF-21d. EELCF-3d. Inoculum EELCF. ELCF-3d. ELCF-21d. Inoculum ELCF. Markers control. Markers control. EELCF-group. Novel Fusobacterium spp. CFV15. Novel Fusobacterium spp. CFV15. Cc. C. innocuum CCI15. Cc. Cc. C. innocuum CCI15. Cl. E. faecalis CEF15.
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A B EELCF-21d EELCF-3d Inoculum EELCF ELCF-3d ELCF-21d Inoculum ELCF Markers control Markers control EELCF-group Novel Fusobacterium spp. CFV15 Novel Fusobacterium spp. CFV15 Cc C. innocuum CCI15 Cc Cc C. innocuum CCI15 Cl E. faecalis CEF15 L. intestinalis CLI15 Cl E. coli CEC15 E. faecalis CEF15 L. intestinalis CLI15 ELCF-group Bact Bif Figure S7: Presence of the strains after inoculation of gnotobiotic rats. (A) PCR-temporal temperature gradient gel electrophoresis (TTGE) was performed according to (Joly et al., 2010). TTGE profiles of cecal contents of EELCF and ELCF rats euthanized 3 and 21 days after inoculation were compared to those of the inoculum. Cc: C. coccoides; Cl: C. leptum; Bact: Bacteroides; Bif: Bifidobacterium. (B) Representative photographs of FISH results from EELCF- and ELCF-group after transfer into ex-GF rats. Scale bar: 20µm. Joly F, Mayeur C, Bruneau A, Noordine ML, Meylheuc T, Langella P et al. (2010). Drastic changes in fecal and mucosa-associated microbiota in adult patients with short bowel syndrome. Biochimie 92:753-761.