1 / 21

IVOS & CEROS Computer Assisted Sperm Analysis

IVOS & CEROS Computer Assisted Sperm Analysis. Spermatology 2006. What is CASA?. An automated system designed to: Visualize and digitize successive images of sperm Process and analyze the images Provide precise and meaningful information on sperm kinematics and counts

bairn
Download Presentation

IVOS & CEROS Computer Assisted Sperm Analysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. IVOS & CEROSComputerAssistedSpermAnalysis Spermatology 2006

  2. What is CASA? • An automated system designed to: • Visualize and digitize successive images of sperm • Process and analyze the images • Provide precise and meaningful information on sperm kinematics and counts • Combination of hardware and software • Hardware: optics, illuminator, digitizer, computer • Software: modules for motility, morphology, and viability analysis

  3. 2 Current CASA Models from HT • IVOS Sperm Analyzer • Built-in optical system • Automated, heated stage • Fastest analysis: 6 fields in 6 seconds • CEROS Sperm Analyzer • Complete system • Includes negative phase contrast microscope

  4. NEW!! Modular CEROS • Choose onlythe options you need • Cost-effective • Use your own negative or positivephase contrast microscope • Same proven and trustedanalysis algorithms

  5. Multi-species Compatibility • Human • Non-human primates • Domestic / companion animals • Production animals • Endangered species • Laboratory rodents • Sea mammals and fish

  6. Customized CEROS for Fish Sperm • “Cooled” stage for optimum maintenance of fish sperm motility • Applications include: • Monitoring sperm motility at hatcheries • Testing extension media and spermiation stimulant • Monitoring environmental pollutants • Reproductive toxicology • Fish, sea urchin, etc.

  7. Negative Phase Contrast - Standard 10x Negative Phase 20x Negative Phase Provides fastest analysis Provides superb visualization

  8. Getting Ready for Analysis (IVOS) • Load sample in fixed or known depth chamber (e.g. Leja Chamber, Microcell, Makler) • Place filled chamber on stage • Select multiple analysis fields • Press Start Scan

  9. Sperm Analysis • Captures multiple “snapshots” extremely quickly (e.g., 30 images in 0.5 second). • Tracks the motile cells from snapshot to snapshot • Static sperm are identified based on motile cell means (size, contrast, intensity) • Computer calculates results

  10. Results • Motility • Progressive Motility • Concentrations • Counts • Velocity - 3 types • Motion parameters

  11. Playback for Quality Control • Motile sperm marked with green track • Progressive sperm marked with blue track • Slow sperm marked with purple track • Static sperm marked with red dot

  12. Motile Playback Sample

  13. Research Options • Obtain data on individual tracks (Edit Tracks) • Study sub-populations of sperm (Sort)

  14. Data Management • Clinical Filing • Custom designed reports • Store reports to disk • HDATA ASCII Export • Export data in database / spreadsheet compatible format • Summary and individual track data

  15. Digital Image Storage • Ability to save all fields analyzed • Recall and reanalyze fields • Sequential field analysis – all fields from original analysis automatically analyzed in order • Share images with other IVOS/CEROS users • Create QC images for training purposes

  16. Morphology Options (Automated) • Dimensions Morphology • Human sperm only • Analysis based on Dr. Kruger’s Strict Criteria • Diff-Quik and PAP stains • Metrix Morphology • Multi-species • Analysis based on user’s definition of normal • Diff-Quik, PAP and other high contrast stains

  17. Real-Time Morphology (non-automated) • Subjective (visual classification) • Fast – “REAL-TIME” • Use non-stained, immobilized sperm, or standard stained smear • Results combined with motility results • Morph-merge option

  18. IDENT Fluorescence (IVOS Only) • Static and motile sperm identification for high-detritus samples • Sperm stained with Hoechst 33342 stain • All sperm fluoresce under UV light • Accurate counts to within 1%

  19. VIADENT Fluorescence (IVOS Only) • Sperm stained with Hoechst 33258 stain • Only NON-VIABLE sperm fluoresce under UV illumination • Counting • Motile/Total counts under standard illumination • Viable count under fluorescent illumination • Only system to perform motility and viability analysis on the same, live sample

  20. Benefits of CASA • Objectivity: Removes technician bias • Accuracy: Best method for determining percent motility, percent progressive motility, and velocity parameters • Precision: Repeatable results • Sensitivity: Detects even the slightest changes in sperm motion • Reliability: Does not get “tired eyes” – can analyze samples all day without sacrificing accuracy

  21. Hamilton Thorne Contact Information • Phone: 978-921-2050, 800-323-0503 • Fax: 978-921-0250 • E-mail: info@hamiltonthorne.com • Web: www.hamiltonthorne.com • 100 Cummings Center, Suite 465EBeverly, MA 01915 USA

More Related