Light Units

1. 40 35 Light Units 30 25 20 15 10 5 0 Auxin/HP HP/HP water/HP 200 Light Units 150 100 50 0 Auxin/HP HP/HP water/HP Supplementary Figure S1 A GSTF8::LUC Expression of GSTF8::LUC but not CaMV35S::LUC construct is regulated by desensitization GSTF8::LUC (A) or 35S::LUC (B) transgenic lines were pretreated for 18 h with either 1mM auxin, 1mM H2O2 (HP) or water, prior to treatment with 1mM H2O2. The bioluminscence of individual seedlings was measure 6 h after the second treatment and plotted. The last column contains statistical analysis using all pair tukey-kramer at 0.05 significance. Non-overlapping circles represent significant differences between treatment. B 35S::LUC

2. mM H2O2 0 10 50 100 300 600 1000 1st Induction Supplementary Figure S2 2nd Induction 1mM H2O2 Only H2O2 concentrations capable of inducing the GSTF8 promoter can activate desensitization Plates containing three rows of four-day GSTF8::LUC seedlings were pretreated with different concentrations of H2O2 14 h prior to 1mM H2O2. For each treatment, 3mL was added to a 55 mm MS plate containing 50mM luciferin and decanted after 40 min. The bioluminescence was measured for each seedling 6 h after after 1st and 2nd treatment using a CCD camera. The purple images reflect the fluorescence of the chlorophyll, while the green represents the luminescence expression from the active GSTF8::LUC construct.

3. 30 25 water 20 1mM auxin Light Units/seedling 1mM OA 15 10 5 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 h Supplementary Figure S3 Okadaic acid treatment does not induce the GSTF8 promoter Four day old GSTF8::LUC seedlings were treated with 1 mM auxin, 1 mM okadiac aicd (OA) or water for 40 min and the bioluminescence over a 19 h time course was measrued using a CCD camera. The average light units per seedling for each treatment, with standard errors, are presented.