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Michaelis-Menten Equation

Maud Menten. V max S. V o = . K m + S. Michaelis-Menten Equation. again, a rectangular hyperbola. rh. V max S. V o = . K m + S. Michaelis-Menten Equation. when there is no S, V 0 is also 0 . as S gets big, V 0 approaches V max. saturable. rh. fig 6-11.

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Michaelis-Menten Equation

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  1. Maud Menten VmaxS Vo = Km + S Michaelis-Menten Equation again, a rectangular hyperbola rh

  2. VmaxS Vo = Km + S Michaelis-Menten Equation when there is no S, V0 is also 0 as S gets big, V0 approaches Vmax saturable rh

  3. fig 6-11

  4. Lineweaver-Burk: 1/S vs. 1/V box 6-1 hyperbola becomes a line

  5. Km + S 1/Vo = VmaxS Lineweaver-Burk Plot rh M & M inverted

  6. Lineweaver-Burk: 1/S vs. 1/V box 6-1 hyperbola becomes a line

  7. In class I show a lot of fancy uses of the LB plot, but you just need to know the basic double reciprocal plot!!!

  8. Lineweaver-Burk: 1/S vs. 1/V box 6-1 hyperbola becomes a line

  9. BIOENERGETICS or Free energy and life

  10. part II intro-1

  11. part II intro-3

  12. part III intro-4

  13. patterns in metabolism there are many…

  14. THE STORAGE and USE of CHEMICAL ENERGY in CELLULAR METABOLISM

  15. “delta gee”- The change in free energy DG = DH - TDS Gibbs free energy enthalpy (heat) entropy (order)

  16. entropy from increased numbers

  17. ? - 1 atm pressure - temperature 25oC - water at 55M - pH = 7 • from initial concentrations of 1M

  18. K’eq is predicted by DG’o… table 13-3

  19. A 10-fold change in Keq for each 1.4 kcal/mole table 13-2

  20. concentration ratio Actual free energy for a reaction when substrates are high- negative when products are high- positive

  21. concentration ratio Actual free energy for a reaction Henri-Louis Le Chatlier

  22. adenosine triphosphate… ATP

  23. adenosine triphosphate… ATP The 20$ bill of the cell!!!

  24. table 13-4

  25. Hydrolysis of ATP fig 13-11

  26. fig 13-11

  27. table 13-6

  28. fig 13-9

  29. [ADP][Pi] [ATP] in erythrocyte Actual ATP hydrolysis energy standard free energy= -30.5 kJ/mole actual free energy= -51.8 kJ/mole

  30. free energies are additive! pg 494

  31. spontaneity determined by whole set of reactions fig 13-11

  32. What is needed to lift this 3 kg dog into the air? Something heavier than 3 kg!!!

  33. 11kg 68kg In metabolism, it is all about the coupling mechanism free energies are additive! riddle: how do you lift 11 kg? ans: lower a weight > 11kg!

  34. spontaneity determined by whole set of reactions (fig 13-11 ed 4)

  35. a mechanism of energy coupling fig 13-8

  36. C.R.H. Raetz Much of metabolism is about LEAVING GROUPS!!! fig 13-8

  37. fig 13-10

  38. adding a good leaving group to form a sigma bond… palmitoyl-CoA + H2O palmitate + CoA-SH This reaction is spontaneous in this direction … so how do we make run the reaction backwards? Ans: couple it to the hydrolysis of ATP

  39. making a good leaving group to form a sigma bond…

  40. driving fancy chemistry by… making a good leaving group! box 13-2

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