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John A.G. Briggs Retrovirus structure and assembly

John A.G. Briggs Retrovirus structure and assembly. The structural genomics of HIV. Gag. Immature virion. Immature HIV. p17. p24. p7. p2. p1. Gag cleavage – can be inhibited. maturation. MMTV. Immature virion. HIV. 2 pathways of Retrovirus formation. Immature virion.

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John A.G. Briggs Retrovirus structure and assembly

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  1. John A.G. Briggs Retrovirus structure and assembly

  2. The structural genomics of HIV

  3. Gag

  4. Immature virion

  5. Immature HIV

  6. p17 p24 p7 p2 p1 Gag cleavage – can be inhibited

  7. maturation

  8. MMTV Immature virion HIV 2 pathways of Retrovirus formation.

  9. Immature virion Internal scaffold domain Cytoplasmic retention motif

  10. MMTV Immature virion HIV Envelopment?

  11. cytosol

  12. cytosol

  13. maturation

  14. Finch/Sundquist lab

  15. Core formed from ~1200 capsid proteins Arranged in ~96Å hexagonal lattice

  16. What is the structure of the lattice here? maturation

  17. Immature HIV-1 In-vitro assembled

  18. If it is made from 80A hexagons, with six proteins per hexagon then 3400 ±200 gag proteins

  19. Scanning Transmission Electron Microscopy Mean mass: 165 ±29MDa 3800 ± 700 gag proteins

  20. 145nm immature virion formed from ~5000 Gag proteins Arranged in ~80 Å hexagonal lattice

  21. Immature virion Tighter hexagonal lattice with 8 nm unit cell. ~5000 gag proteins form a complete shell for a 145 nm virus Mature virion Looser hexagonal lattice with 9.6 nm unit cell. ~1200 CA proteins form the conical core, leaving a large number elsewhere

  22. Virology University of Heidelberg University of Oxford Cornell University Hans-Georg Kraüsslich Barbara Müller Marko Lampe Bärbel Glass Marc Johnson Volker Vogt Quentin Sattentau Electron Microscopy University of Oxford Max-Planck Institute for Biochemistry Stephen Fuller Kay Grünewald Friedrich Förster Thomas Wilk Giulia Zanetti

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