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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

GEIL-GTLLF 2008. Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies. Part three : Monocytic maturation. III.1. SSC. SSC. SSC. SSC. SSC. SSC. FLy. FLy. FLy. FLx. CD45. FLn. CD45. FLx. CD45. FLx. CD45. CD45. Monober. Backgating.

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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

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  1. GEIL-GTLLF 2008 Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies Part three : Monocytic maturation III.1

  2. SSC SSC SSC SSC SSC SSC FLy FLy FLy FLx CD45 FLn CD45 FLx CD45 FLx CD45 CD45 Monober Backgating Strategy Display III.2

  3. SSC SSC SSC SSC SSC FL3 FL3 FL2 CD45 CD45 CD45 FL2 FL1 FL1 CD45 CD45 FL2+ FL1+ FL3+ Crossgating SSC FLn Strategy Display III.3

  4. Informative combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 5 : CD38/DR/CD34/CD45 CD11b/CD14 p. III.5 CD38/HLADR p. III.21 CD11b/CD16 p. III.6 CD38/CD34 p. III.22 CD14/CD16 p. III.7 HLADR/CD34 p. III.23 Crossgating p. III-8 Crossgating p. III.24 Tube 2 : CD11b/CD117/CD34/CD45 Tube 7 : DR/CD117/CD34/CD45 CD11b/CD117 p. III-9 HLADR/CD117 p. III.25 CD11b/CD34 p. III-10 HLADR/CD34 p. III.26 CD117/CD34 p. III-11 CD117/CD34 p. III.27 Crossgating p. III-12 Crossgating p. III.28 Tube 3 : CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD36/CD71 p. III-13 CD64/CD24 p. III.29 CD36/CD11c p. III.14 CD64/CD10 p. III.30 CD71/CD11c p. III.15 CD24/CD10 p. III.31 Crossgating p. III.16 Crossgating p. III.32 Tube 4 : CD34/CD13/CD33/CD45 CD34/CD13 p. III.17 CD34/CD33 p. III.18 CD13/CD33 p. III.19 Crossgating p. III.20 IIII.4

  5. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD14PE CD14PE CD14PE CD11bFITC CD11bFITC CD11bFITC CD11b/CD14 Gating CD14 Monober Gating CD11b III.5

  6. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD16PC5 CD16PC5 CD16PC5 CD11bFITC CD11bFITC CD11bFITC CD11b/CD16 Gating CD16 Monober Gating CD11b III.6

  7. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD16PC5 CD16PC5 CD16PC5 CD14PE CD14PE CD14PE CD14/CD16 Gating CD16 Monober Gating CD14 III.7

  8. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45ECD CD45ECD CD45ECD CD16PC5 CD16PC5 CD14PE CD11bFITC CD11bFITC CD14PE Crossgating CD11b/CD14/CD16 Gating CD 16 Gating CD11b Gating CD14 III.8

  9. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45APC CD45APC CD45APC CD117PE CD117PE CD117PE CD11bFITC CD11bFITC CD11bFITC CD11b/CD117 Gating CD117 Monober Gating CD11b III.9

  10. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45APC CD45APC CD45APC CD34PC7 CD34PC7 CD34PC7 CD11bFITC CD11bFITC CD11bFITC CD11b/CD34 Gating CD34 Monober Gating CD11b III.10

  11. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45APC CD45APC CD45APC CD34PC7 CD34PC7 CD34PC7 CD117PE CD117PE CD117PE CD117/CD34 Gating CD34 Monober Gating CD117 III.11

  12. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45APC CD45APC CD45APC CD34PC7 CD34PC7 CD117PE CD11bFITC CD11bFITC CD117PE Crossgating CD11b/CD117/CD34 Gating CD11b Gating CD 34 Gating CD117 III.12

  13. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD71PE CD71PE CD71PE CD36FITC CD36FITC CD36FITC CD36/CD71 Gating CD71 Monober Gating CD36 III.13

  14. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD11cPC5 CD11cPC5 CD11cPC5 CD36FITC CD36FITC CD36FITC CD36/CD11c Gating CD11c Monober Gating CD36 III.14

  15. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD11cPC5 CD11cPC5 CD11cPC5 CD71PE CD71PE CD71PE CD71/CD11c Gating CD11c Monober Gating CD71 III.15

  16. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45ECD CD45ECD CD45ECD CD11cPC5 CD11cPC5 CD71PE CD36FITC CD36FITC CD71PE Crossgating CD36/CD71/CD11c Gating CD11c Gating CD36 Gating CD71 III.16

  17. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD13PE CD13PE CD13PE CD34FITC CD34FITC CD34FITC CD34/CD13 Gating CD13 Monober Gating CD34 III.17

  18. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD33APC CD33APC CD33APC CD34FITC CD34FITC CD34FITC CD34/CD33 Gating CD33 Monober Gating CD34 III.18

  19. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD33APC CD33APC CD33APC CD13PE CD13PE CD13PE CD13/CD33 Gating CD33 Monober Gating CD13 III.19

  20. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45PC7 CD45PC7 CD45PC7 CD33APC CD33APC CD13PE CD34FITC CD34FITC CD13PE Crossgating CD34/CD13/CD33 Gating CD33 Gating CD34 Gating CD13 III.20

  21. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD HLADRPE HLADRPE HLADRPE CD38FITC CD38FITC CD38FITC CD38/HLADR Gating HLADR Monober Gating CD38 III.21

  22. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD38FITC CD38FITC CD38FITC CD38/CD34 Gating CD34 Monober Gating CD38 III.22

  23. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 HLADRPE HLADRPE HLADRPE HLADR/CD34 Gating CD34 Monober Gating HLADR III.23

  24. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45ECD CD45ECD CD45ECD HLADRPE CD34PC5 CD34PC5 CD38FITC CD38FITC HLADRPE Crossgating CD38/HLADR/CD34 Gating CD 34 Gating CD38 Gating DR III.24

  25. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD117PE CD117PE CD117PE HLADRFITC HLADRFITC HLADRFITC HLADR/CD117 Gating CD117 Monober Gating HLADR III.25

  26. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 HLADRFITC HLADRFITC HLADRFITC HLADR/CD34 Gating CD34 Monober Gating DR III.26

  27. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD117PE CD117PE CD117PE CD117/CD34 Gating CD34 Monober Gating CD117 III.27

  28. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD117PE HLADRFITC HLADRFITC CD117PE Crossgating HLADR/CD117/CD34 Gating CD 34 Gating HLADR Gating CD117 III.28

  29. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD24PE CD24PE CD24PE CD64FITC CD64FITC CD64FITC CD64/CD24 Gating CD24 Monober Gating CD64 III.29

  30. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD10PC5 CD10PC5 CD10PC5 CD64FITC CD64FITC CD64FITC CD64/CD10 Gating CD10 Monober Gating CD64 III.30

  31. SSC SSC SSC CD45 CD45 CD45 FLy FLy FLy FLx FLx FLx SSC-Height SSC-Height SSC-Height CD45PC7 CD45PC7 CD45PC7 CD10PC5 CD10PC5 CD10PC5 CD24PE CD24PE CD24PE CD24/CD10 Gating CD10 Monober Gating CD24 III.31

  32. SSC SSC SSC CD45 CD45 CD45 FL3+ FL2+ FL1+ FL3 FL3 FL2 SSC-Height SSC-Height SSC-Height FL1 FL1 FL2 CD45PC7 CD45PC7 CD45PC7 CD10PC5 CD10PC5 CD24PE CD64FITC CD64FITC CD24PE Crossgating CD64/CD24/CD10 Gating CD10 Gating CD64 Gating CD24 III.32

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