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A Day in the Lab with Ms. Marguerite McDonald: Biochemist & Grad Student

Join Ms. Marguerite McDonald as she delves into Enzymology at Dr. Mueller’s Mechanistic Enzymology Lab, University of Delaware. Explore the central dogma of biochemistry and the chemistry behind pseudouridine synthases. Witness the isomerization of RNA and the purification processes involved. Delve deep into the world of protein, RNA, and DNA through this fascinating journey.

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A Day in the Lab with Ms. Marguerite McDonald: Biochemist & Grad Student

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  1. A Day in the Lab with... Name:Ms. Marguerite McDonaldTitle: Biochemist, grad studentConcentration: EnzymologyWorkPlace : Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department University of Delaware Graduate Program

  2. PROTEIN RNA DNA The Central Dogma Biochemistry http://bioweb.wku.edu/courses/BIOL115/Wyatt/Nucleic_Acids/mRNA2.htm

  3. The study of the action (the mechanism) of an enzyme  synthase Pseudouridine synthases Mechanistic Enzymology http://www.sdnhm.org/exhibits/epidemic/teachers/images/bacteria.jpg

  4. O O H N H N N O O N The Chemistry: Isomerization of RNA

  5. TruA’s substrate: transfer RNA

  6. Purifying radioactive tRNA and TruA SDS-polyacrylamide gel showing pure TruA and TruA before purification urea-polyacrylamide gel showing pure tRNA and tRNA with DNA contamination

  7. Questions? O H H N N O

  8. H N O O O O N O O O H H N N N O N H H N N N O O

  9. H N O O O O O O N O O O H H N N N O N H H N N N O H N O N Pseudouridine Synthases

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