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Determining Ruminant Materials In Feed Ingredients. Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas 77841. ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas. Microscopy Equipment. Compound Microscope Steroscopic Microscope
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Determining Ruminant Materials In Feed Ingredients Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas 77841 ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas
Microscopy Equipment • Compound Microscope • Steroscopic Microscope • Reagents for Spot Tests • Accessories
Microscopic Procedures • Use a 20 gram Sample for Viewing with the Stereomicroscope • Take a Separate 10 gram Sample for Floatation • Observe Mineral Portion • Spot Tests for Confirmation
Examples of Spot Testing • 10% HC1 for Carbonates, Limestone, or Oyster Shell • Nesslers Reagent for Ammonium Containing Compounds • Urea Spot Testing • Silver Nitrate for Chlorides • Quimociac for Phosphates NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent
Application of Microscopy • Levels of Meat and Bone in Feed Sample • Identify Milk and Dried Blood • Microscopy is a Rapid Procedure • OTSC Current Surveys
Agri-Screen by Neogen Corporation • Neogen has Two Test Strips • Presence of Ruminant Meat and Bone in Animal Protein Products • Presence of Ruminant Meat and Bone in Feeds
Agri-Screen Procedures for Ruminant M&B Meal • Weigh 10 grams Sample in Mason Type Jar • Add 100 ml Extraction Solution • Place in Boiling Water Bath for 10 Minutes • Remove Mason Jar from Water Bath • Prepare Sample Tubes and Transfer 0.5 ml of Extract to a Tube (Use different pipette for each sample) • Place Sample Strip into each Sample Tube • Develop for 10 Minutes and Read Results
Reading Strip Results • Negative-Control Line Appears Only • Postive-Control Line and Sample Line Appear