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Determining Ruminant Materials In Feed Ingredients

Determining Ruminant Materials In Feed Ingredients. Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas 77841. ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas. Microscopy Equipment. Compound Microscope Steroscopic Microscope

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Determining Ruminant Materials In Feed Ingredients

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  1. Determining Ruminant Materials In Feed Ingredients Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas 77841 ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas

  2. Microscopy Equipment • Compound Microscope • Steroscopic Microscope • Reagents for Spot Tests • Accessories

  3. Microscopic Procedures • Use a 20 gram Sample for Viewing with the Stereomicroscope • Take a Separate 10 gram Sample for Floatation • Observe Mineral Portion • Spot Tests for Confirmation

  4. Spot Testing Reagants

  5. Examples of Spot Testing • 10% HC1 for Carbonates, Limestone, or Oyster Shell • Nesslers Reagent for Ammonium Containing Compounds • Urea Spot Testing • Silver Nitrate for Chlorides • Quimociac for Phosphates NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent

  6. Application of Microscopy • Levels of Meat and Bone in Feed Sample • Identify Milk and Dried Blood • Microscopy is a Rapid Procedure • OTSC Current Surveys

  7. Meat and Bone in Feed

  8. Meat and Bone Meal in Feed

  9. Meat and Bone Meal in Feed

  10. Meat and Bone Meal in Feed

  11. Bone Fragment

  12. Animal Bone in Feed

  13. Blood Meal in Feed

  14. Meat and Bone Meal

  15. Poultry Meal

  16. Feather Meal

  17. Hydrolyzed Feathers

  18. Agri-Screen by Neogen Corporation • Neogen has Two Test Strips • Presence of Ruminant Meat and Bone in Animal Protein Products • Presence of Ruminant Meat and Bone in Feeds

  19. Agri-Screen Procedures for Ruminant M&B Meal • Weigh 10 grams Sample in Mason Type Jar • Add 100 ml Extraction Solution • Place in Boiling Water Bath for 10 Minutes • Remove Mason Jar from Water Bath • Prepare Sample Tubes and Transfer 0.5 ml of Extract to a Tube (Use different pipette for each sample) • Place Sample Strip into each Sample Tube • Develop for 10 Minutes and Read Results

  20. Reading Strip Results • Negative-Control Line Appears Only • Postive-Control Line and Sample Line Appear

  21. Discussion

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