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申明华 制药工程

Metabolic engineering of xylose metabolic pathway optimization. 申明华 制药工程. Bärbel Hahn- Hägerdal , born in 1943, was appointed professor of Applied Microbiology 1990 and is now emerita professor at the division of Applied Microbiology. .

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申明华 制药工程

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  1. Metabolic engineering of xylose metabolic pathway optimization 申明华 制药工程

  2. Bärbel Hahn-Hägerdal, born in 1943, was appointed professor of Applied Microbiology 1990 and is now emerita professor at the division of Applied Microbiology. Her work initially focused on the enzymatic hydrolysis of cellulose, but has in the past 15 years turned to the fermentation of lignocellulosic hydrolysates with recombinant strains of baker’s yeast Saccharomyces cerevisiae.

  3. Aims & scope Biotechnology for Biofuels is an open access, peer-reviewed online journal featuring high-quality studies describing technological and operational advances in the production of biofuels from biomass. www.biotechnologyforbiofuels.com

  4. Ethanol PPP

  5. stable for more than 40 generations TMB3001 S. Cerevisiae CEN.PK 113-7A XR, XDH, and XK grew on xylose only in the presence of oxygen Anaerobic ethanol formation from xylose by recombinant S. cerevisiae was demonstrated for the first time.

  6. Strain construction XR XDH XK chromosomal integration TMB 3399 S. Cerevisiae USM21 chemical mutagenesis XR XDH XK TMB 3400

  7. Xylitol Xylitol

  8. metabolic engineering random mutagenesis Higher growth rate Lower xylitol yield ……

  9. XI XK PPP ΔGRE3 XI XK PPP ΔGRE3 repeated cultivation on xylose medium TMB3045 TMB3050

  10. 本文中对菌株的改造 主要通过一下几个方面： 1 GRE3的敲除 2 下游XKS及PPP途径的加强 3 外源XR-XDH途径的引入

  11. XR-XDH，多拷贝游离型质粒pY7 TMB3057 TMB3044 XI，多拷贝游离型质粒YEpHXT TMB3066

  12. Aerobic growth

  13. TMB3057 Anaerobic xylose fermentation TMB3400 TMB3066

  14. Fermentation of lignocellulosehydrolysate

  15. 对XDH的定向进化 XR XDH Xylose Xylitol Xylulose NADP+ NADPH NADP+ NAD+ NADH NADPH 207D 208I 209F 211N 207A 208R 209S 211R XDH XDH Watanabe, S., Kodaki, T., and Makino, K. J. Biol. Chem., 2005,280, 10340-10349.

  16. Cellulosic biomass Pretreatment Xylose Hemicellulose Pretreatment xylose XR XI Upstream module xylitol XDH Cellulose xylulose XKS1 5P-xylulose Glucose Downstream module PPP Ethanol Glycolysis Pyruvate

  17. THANK YOU!

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