Making sense of a gene fusion event in Proteobacteria

Making sense of a gene fusion event in Proteobacteria Dawn Anderson (Berea College) Rob Barber (U of Wisconsin Parkside) Teresa Johnson (College of Wooster)

A promiscuous protein domain (and coding region) The GAT-1 family is comprised of proteins containing a Type-1 glutamine amidotransferase-like domain. Proteins in this family display an alpha/beta hydrolase fold with a nucleophilic elbow. This transferase-like domain can be present alone or as a fusion with several other protein domains. A fusion between this domain and an AraC DNA binding domain has been found in prokaryotes.

Domain fusion to ArgF DNA binding domain restricted to Proteobacteria

Phylogeny suggests divergence prior to speciation

N-terminal domain phylogeny

Gene associations/neighborhoods offer little functional inference

Approaches to test gene product function • Clone gene, express and purify predicted gene product (trying heparin chromatography) • Structure determination and model building exercises • Gene expression experiments possible (microarrays, proteomics) but likely offer little insight due to prediction of regulatory role

FusionDB offered little insight regarding this protein family

Making sense of a gene fusion event in Proteobacteria

Making sense of a gene fusion event in Proteobacteria

Presentation Transcript

Making Sense of Division

Making sense of a MAD experiment.

Making Sense of Middleware

SENSE-MAKING .

of making sense...

Making Sense of Writing

Making Sense of Smells

Making Sense of Nonfiction

Making Sense of Mathematics

Making sense of records

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Making Sense of a History Monograph

Making Sense of Culture

Proteobacteria

Making sense of voices

Making Sense of History

Making sense of SaaS

Making Sense of Maps

Making Sense of Models

Making Sense of Genomes

of making sense...

MAKING SENSE OF HISTORY: