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Laboratory Methods in Biotechnology

Laboratory Methods in Biotechnology. Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety. Idea Behind the Course.

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Laboratory Methods in Biotechnology

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  1. Laboratory Methods in Biotechnology Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety

  2. Idea Behind the Course • Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses. • Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions.

  3. Role in Your Curriculum Core: Reasoning, Investigation, Cell Biology, Genetics, MCMB Specialty: Lab Methods, Micro, Molecular Genetics Electives: Molecular Techniques, Cell and Molec, Immunology, Path Micro, Microbial Phys, etc

  4. Objectives • To give students experience in dimensional analysis used in microbiology and cellular and molecular biology, and biotechnology

  5. Objectives • To familiarize the students with the theory and practice of preparing solutions from various starting materials.

  6. Objectives • To introduce the students to means of preparing sterile media and solutions.

  7. Objectives • To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations.

  8. Objectives • To allow students to quantify proteins, nucleic acids and other chemicals in solution.

  9. Objectives • To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc.

  10. Objectives • To introduce the students to the use of enzymes for end-point determination and to the measurement of enzyme activity.

  11. Objectives • To give students experience in the techniques for the manipulation and electrophoretic separation of proteins and DNA.

  12. Objectives • To provide information regarding biological, chemical, radiation, and other aspects of lab safety.

  13. Textbooks • Make sure you bring this each time. • Seidman and Moore – our main text • Leboffe and Pierce – illustration of techniques • Also need: • Calculator! • Lab Notebook

  14. Additional Resources • Brock – background information on theory • Day – advice on writing (humor) • Robyt - Source of many handouts/additional readings • CBE Manual – Style guide for preparation of manuscripts • Don’t buy these

  15. Webpagehttp://cstl-csm.semo.edu/champine/BI245/index.html • Lab Activities • Lecture Topics • Homework Assignments • In Class Assignments • Manuscripts • Notebook Guidelines • Syllabus • Grade Book

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