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Padova sequencing contribution: 100,000 Fosmid ends 4-5 runs 454-Titanium

Padova sequencing contribution: 100,000 Fosmid ends 4-5 runs 454-Titanium 2 slides SOLiD (10Kb inserts, 50x2 bases) ‏. Data production. Mapping SOLiD reads on 454 contigs. Sorting out the links. Counting links to produce SOLID arches. 4 possible ways to merge 2 fragments.

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Padova sequencing contribution: 100,000 Fosmid ends 4-5 runs 454-Titanium

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  1. Padova sequencing contribution: 100,000 Fosmid ends 4-5 runs 454-Titanium 2 slides SOLiD (10Kb inserts, 50x2 bases)‏

  2. Data production

  3. Mapping SOLiD reads on 454 contigs

  4. Sorting out the links

  5. Counting links to produce SOLID arches 4 possible ways to merge 2 fragments Count all possible merging betweenall the possible pairs of contigs

  6. Building a path to order the contigs List of links (arches)‏ 1: 9, 12 2: 6,18 3: 17, 20 4: 5 5: 4, 15 6: 2, 16 7: 14, 10 8: 11, 19 9: 1, 16 10: 7 11: 8, 15 12: 1, 17 13: 14, 20 14: 7, 13 15: 5, 11 16: 6, 9 17: 3, 12 18: 2, 19 19: 8, 18 20: 3, 13 From the above list it should be easily produced the following scaffold: 4 - 5 - 15 - 11 - 8 - 19 - 18 - 2 - 6 - 16 - 9 - 1 - 12 - 17 -3 – 20 – 13 – 14 – 7 - 10

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