Biological importance of Osmotic pressure

1. Biological importance of Osmotic pressure 29.11.12

2. Osmosis takes place due to difference in hydrostatic pressure of water and solution separated by a semi permeable membrane (permeable only to water)

3. Measurement of Osmotic Pressure • Apply an external pressure to the side containing solution • The hydrostatic pressure which just stops osmosis is the osmotic pressure of the solution • Osmotic pressure is the excess of the pressure required to equalize water activities in the two compartments

4. Osmosis when two solutions with different hydrostatic and osmotic pressure are separated by a semi permeable membrane The difference in net hydrostatic pressures of two solutions is equal to difference in osmotic pressures of two solutions

5. Water is pulled into the solution with relatively higher pulling tendency (concentrated solution). Osmosis takes place

6. Reverse Osmosis Reverse osmosis is a membrane based filtration method that removes many types of large molecules and ions from solutions by applying pressure to the solution when it is on one side of a selective membrane. If an external pressure is applied on a concentrated solution, this pressure is distributed evenly throughout the solution If the applied pressure is higher than the osmotic pressure water will flow towards the other side of the membrane leaving solute behind This technique is used for purification of water

7. Reverse Osmosis

8. Importance of Osmosis and Osmotic Pressure Oncotic pressure of blood plasma Formation of tissue fluid Regulation of cell volume

9. Oncotic pressure of blood plasma • Some 90% by weight of plasma is water and about 8% is plasma proteins (albumin, globulins, fibrinogens) • Blood plasma is an aqueous solution containing different ions (Na+, K+, Ca2+…), small non dissociated molecules (glucose, amino acids) and proteins- macromolecules (albumin, globulin etc) • Each type of molecules contributes with its own osmotic pressure, the sum representing the colloid-osmotic pressure or oncotic pressure of plasma πplasma = ∑ πmol + ∑ πions+ ∑ πproteins

10. Oncotic pressure of blood plasma • Albumin is the major contributor to oncotic pressure of plasma because it has the lowest molecular weight of the major plasma proteins and its concentration is almost double that of globulin • The total oncotic pressure of an average capillary is about 28 mmHg with albumin contributing approximately 22 mmHg of this oncotic pressure. • Throughout the body, dissolved compounds have an osmotic pressure.

11. Because large plasma proteins cannot easily cross through the capillary walls, their effect on the osmotic pressure of the capillary interiors will, to some extent, balance out the tendency for fluid to leak out of the capillaries. In other words, the oncotic pressure tends to pull fluid into the capillaries.

12. Tissue Fluid Formation Filtration takes place at the arterial end of capillary because hydrostatic pressure of blood overcomes the oncotic pressure of plasma proteins Reabsorption takes place at the venous end of capillary because hydrostatic pressure of blood falls below the oncotic pressure of plasma proteins Net result of this filtration/ultrafiltration and reabsorption/osmosis at arterial and venous end of capillary is the tissue fluid formation

13. Starling Equation • The Starling equation is an equation that illustrates the role of hydrostatic and oncotic forces (the so-called Starling forces) in the movement of fluid across capillary membranes

14. Tissue Fluid Formation

15. Tissue Fluid Formation

16. Removal of tissue fluid To prevent a build up of tissue fluid surrounding the cells in the tissue, the lymphatic system plays a part in the transport of tissue fluid. Tissue fluid can pass into the surrounding lymph vessels

17. Edema If the ultrafiltration is excessive, the volume of interstitial fluid increases. When it becomes clinically detectable, it is called edema Venous obstruction or plasma protein deficiency can lead to edema In conditions where plasma proteins are reduced, e.g. from being lost in the urine (proteinuria) or from malnutrition, there will be a reduction in oncotic pressure and an increase in filtration across the capillary, resulting in excess fluid buildup in the tissues

18. Maintenance of Cell Volume The determinants of cell volume are the total number of osmotically active particles within the cell and the osmolarity of the extracellular fluid. The cell has a considerable quantity of impermeant solutes i. e. proteins and organic phosphates whereas the interstitial fluid is relatively devoid of these. Hence there exists a colloid osmotic gradient across the cell and this would draw fluid into the cell. This effect of cell macromolecules is offset by the Na+-K+ pump

19. Maintenance of Cell Volume 3 positive ions (Na+) are pumped out of the cell (towards ECF) for every 2 positive ions (K+) pumped into the cell (towards ICF). This means that there is more positive charges leaving the cell than entering it. As a result, positive charge builds up outside the cell compared to inside the cell. The difference in charge between the outside and inside of the cell limits the fluid flow into the cell. About 90% of the osmotic pressre of extracellular fluid is due to sodium ions

22. Maintenance of body fluid osmolality by Kidney Kidney maintains the optimum osmolality of body fluid by regulating the volume of body fluids When water intake is low or when water is lost through diarrhea or perspiration, the kidney conserves water by producing a small volume of urine which is hypertonic When water intake is high, the kidney excretes a large volume of hypotonic urine. Kidney maintains normal osmolality by regulating excretion of water and sodium chloride within a narrow range

23. Surface Tension • The force with which surface molecules are held is called the surface tension of the liquid • It is the force acting perpendicularly inward on the surface layer of a liquid to pull its surface molecules towards the interior of the fluid • It keeps the surface like a stretched membrane, and hence keeps the contact area minimum

24. Surface Tension • Water striders use surface tension to walk on the surface of pond. The surface of the water behaves like an elastic film: the insect's feet cause indentations in the water's surface. Its tiny mass and geometry of its legs allow it to be supported by the high surface tension of water • Formation of drops occurs when a mass of liquid is stretched. Water adhering to the tap gains mass until it is stretched to a point where the surface tension can no longer bind it to the tap. It then separates and surface tension forms the drop into a sphere. If a stream of water were running from the tap, the stream would break up into drops during its fall. Gravity stretches the stream, then surface tension pinches it into spheres

25. Surface tension at interfaces • Surface tension at liquid-air interface: A soap bubble is a thin film of soapy water enclosing air that forms a hollow sphere. Surface tension causes a bubble to assume the smallest surface area to contain a given volume -- resulting in the spherical shape • Liquid-solid interface Beading of rain water on the surface of a waxy surface, such as a leaf. Water adheres weakly to wax and strongly to itself, so water clusters into drops. Surface tension gives them their near-spherical shape, because a sphere has the smallest possible surface area to volume ratio • Liquid-Liquid interface Separation of oil and water (in this case, water and liquid wax) is caused by a tension in the surface between dissimilar liquids.

26. Reference book • Chapter 3: Human Physiology: From Cells to Systems By Lauralee Sherwood • Chapter 1 :An Introduction to Med. Biophysics  by Prakash • Chapter 10: Biophysics by P. S. Mishra