Zayed Almadidy

1. In Vitro Generation of CuI Through Redox Chemistry And Its Stabilization By Means of Organic Stabilizing Ligands TODAY TOPIC IS WE ARE CURRENTLY ON SLIDE Zayed Almadidy Inorganic Biochemistry 1 of 27 PRESENTER

2. Small INTRO Background on the preparation of CuI • Common three oxidation states: • Cu0 • CuI • CuII • Cu0is most abundant • CuIis difficult to work with under anaerobic and aerobic conditions. Comproportionation CuII+ Cu0D2CuI PLACE IMAGE HERE NOT STABLE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 2 of 27 PRESENTER

3. Instability of Cu1+ Equilibrium constants and reduction potential CuII + e-D CuI ∑o= -.521 V Cu0D CuI + e- ∑o= .153 V Cu0 + CuII D 2CuI ∑o= -.368 V YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 3 of 27 PRESENTER PRESENTER

4. Gibbs Free ENERGY Nernst Equation and Equilibrium Theory Using Equilibrium Theory: There will be 832.31 times more CuII than CuI YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 4 of 27 PRESENTER PRESENTER

5. Stabilizing Cu1+ Utilizing BCA, BCS and MeCN Cu0 + CuIID 2CuI 2CuI + XD 2(CuIX2) LeChatlier’s Principle: Reaction will favor products YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 5 of 27 PRESENTER PRESENTER

6. Ligand BCS Structures of Organic Agents In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 6 of 27 PRESENTER

7. Ligand BCA Structures of Organic Agents In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 7 of 27 PRESENTER

8. Ligand MeCN Structures of Organic Agents In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 8 of 27 PRESENTER

9. Goals of the PROJECT What is the destination of the experiment? Understand kinetics of comproportionation reaction. Acquire a stability constant for the CuII—Ligand complex. To optimize the reaction. PLACE IMAGE HERE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 9 of 27 PRESENTER

10. Experimental PROCEDURE How it was accomplished Used spectrophotometer 10 uL injections of X ligand Data recorded as a function of time 1.5 mL Buffer 1.5 mL H2O X mL CuII PLACE IMAGE HERE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 10 of 27 PRESENTER

11. Accidental FINDING In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 11 of 27 PRESENTER

12. Data ANALYSIS What does it mean? CuII + X D 2CuI CuI + 2BCSD CuIBCS2 483 nm peak indicative of CuI/BCS Complex YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 12 of 27 PRESENTER PRESENTER

13. Electron SOURCE What is the reducing agent? 1. Water 2. Buffer One of these must have been the reducing agent YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 13 of 27 PRESENTER PRESENTER

14. Water HYPOTHESIS How is water yielding electrons? 4 OH-D 2 H2O + O2 + 4 e- ∑o= -.401 V 4(CuX2)2+ + 4e- D4(CuX2)1+ ∑o= .64 V 4(CuX2)2+ + 4 OH-D2 H2O + O2 + 4(CuX2)1+ ∑o= .239 V YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 14 of 27 PRESENTER PRESENTER

15. Testing HYPOTHESIS Altering the pH 4(CuX2)2+ + 4 OH-D2 H2O + O2 + 4(CuX2)1+ LeChatlier’s Principle: Increased hydroxide  Increased products YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 15 of 27 PRESENTER PRESENTER

16. pH 8.3 HEPES In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 16 of 27 PRESENTER

17. pH COMPARISONS In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 17 of 27 PRESENTER

18. Cu0 to Cu1+ How it was accomplished Vial A Vial B Cu0 wire 0.060 mL BCA 0.5 mL MOPS 0.44 mL H2O Cu0 wire .056 mL CuII 0.060 mL BCA 0.5 mL MOPS 0.439 mL H2O PLACE IMAGE HERE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 18 of 27 PRESENTER

19. O2HYPOTHESIS Effect of oxygen on the reaction progression 2Cu0 D2CuI + 2e- • ∑o = - 0.521 V • 2H+ + 2e- + ½O2 DH2O • ∑o = 2.421 V • 2Cu0 + 2H+ + ½ O2D2CuI + H2O • ∑o = 1.900 V Anaerobic Conditions Cu0 wire 1000uM BCA pH 6.5 MOPS H2O In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 19 of 27 PRESENTER

20. Observed RESULTS What did we see? Anaerobic Aerobic Cu0 wire 1000uM BCA pH 6.5 MOPS H2O PLACE IMAGE HERE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 20 of 27 PRESENTER

21. Reaction KINETICS Quantifying stabilization reaction Anaerobic Conditions CuII +Cu0 + MeCND(CuIMeCN) 50 mMCuII Cu0 Various MeCN pH 6.5 MES H2O In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 21 of 27 PRESENTER

22. Rate DEPENDENCE = k[CuII]a[MeCN]b[CuI]c b = 0.754227 ± 0.072543 In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 22 of 27 PRESENTER

23. Cu2+DEPENDENCE In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 23 of 27 PRESENTER

24. Future WORK What’s next? Determine what effect the concentration of CuI has on the kinetics Further optimize the reaction YOU ARE LOOKING AT In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy Winthrop University 3 of 27 24 of 27 PRESENTER PRESENTER

25. The CREW Mentor: Dr. Nicholas Grossoehme Sharon Jenkins Destinee Johnson Becca Toor Paisley Trantham In The Research labs of WE ARE CURRENTLYHERE Zayed Almadidy Winthrop University 25 of 27 PRESENTER

26. Show is over Thank you! YOU ARE LOOKING AT TODAY TOPIS IS In The Research labs of WE ARE CURRENTLYHERE WE ARE CURRENTLYHERE Presenter Name Zayed Almadidy HERE GOES THE TITLE OF THE TOPIC Winthrop University 7 of 27 26 of 27 PRESENTER PRESENTER www.totalydarkpowerpointtemplate.com