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A. 0. 30. 60. min. XKid -. Myf5. DEAE fractions. Control. Control IP. Cdc27 IP. 1. 2. 3. 4. 5. 6. 7. DEAE 0.5M NaCl. DEAE 0.25M NaCl. Control supernatant. Cdc27 beads. Cdc27 supernatant. Control beads. Buffer. B. (Ub) n -cycB. cycB. (Ub) n -Myf5. Myf5.
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A 0 30 60 min. XKid - Myf5 DEAE fractions Control Control IP Cdc27 IP 1 2 3 4 5 6 7 DEAE 0.5M NaCl DEAE 0.25M NaCl Control supernatant Cdc27 beads Cdc27 supernatant Control beads Buffer B (Ub)n-cycB cycB (Ub)n-Myf5 Myf5