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Amino Acids Production

Amino Acids Production. REXIM S.A. Headquarter : Courbevoie , France, (Paris) Production Plants for Pharmaceutical Grade L-amino acids : Ham , France Konstanz , Germany Nanning , China (2001) Production Plants for Feed Grade Aminoacids Blair ,Mobile , USA (L-lys ,DL-met)

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Amino Acids Production

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  1. Amino Acids Production REXIM S.A.

  2. Headquarter : Courbevoie , France, (Paris) Production Plants for Pharmaceutical Grade L-amino acids : Ham , France Konstanz , Germany Nanning , China (2001) Production Plants for Feed Grade Aminoacids Blair ,Mobile , USA (L-lys ,DL-met) Antwerp , Belgium (DL-met ) Wesseling , Germany ( DL-met ) Slovakia ( L-lys ,L-thr ) Rexim is 100% Degussa since 20 years REXIM S.A.

  3. Production Plants for Pharmaceutical Grade L-Amino Acids : Ham , France : Extraction from Proteins hydrolyzates Purification from lower grades ( feed,...) Konstanz , Germany : Enzymatic resolution Nanning , China : Biocatalysis and fermentation Production Plants for Feed Grade Amino acids Synthesis Fermentation Processes REXIM S.A.

  4. Serine , Threonine , Proline , Hydroxyproline, Alanine, Valine, Isoleucine , Leucine Phenylalanine, Histidine, Arginine ( asp, glu, gly, lys, tyr ,met) L-Amino Acids Extraction from Proteins Hydrolysates1/8 REXIM S.A.

  5. Selection Purification of natural Proteins Acidic Hydrolysis Filtration Ion exchange chromatographic steps Charcoal treatment (Ultrafiltration) Crystallization 1 Charcoal treatment Ultrafiltration Crystallization 2 ,Drying Extraction from Proteins Hydrolysates2/8 :Process REXIM S.A.

  6. Selection Purification of natural Proteins and pure Chemicals: Keratins from feathers ( chicken ,ducks ,turkeys ) Gelatin from pigs Bovine free materials Chemicals like NH3, HCl, NaOH, H2SO4,alcohols Regular Suppliers Audits Extraction from Proteins Hydrolysates3/8 REXIM S.A.

  7. Acidic Hydrolysis of Proteins: Conditions choosen for complete disappearance of proteinic materials : only short peptides (2-4 units ) remain. Use of a large excess of concentrated HCl. Keratin is heated 6 hrs at 105°C Gelatin is heated 4 hrs at 120°C Extraction from Proteins Hydrolysates4/8 REXIM S.A.

  8. Ion exchange chromatography : Main step achieving separation of single amino acids. Separation based on ionic properties but also on differences on sizes and hydrophobicities ( peptides separated aswell) Extraction from Proteins Hydrolysates5/8 REXIM S.A.

  9. Activated mineral Charcoal treatment : Purification based on surface adsorption : Charcoal fixes coloured entities and macromolecules ( endotoxines... ) Extraction from Proteins Hydrolysates6/8 REXIM S.A.

  10. Ultrafiltration : Main Purpose is biological purification : removal of endotoxines for making infusion grade amino acids . Done on membranes with cut off of 5000 to 10 000 daltons; Efficiency measured by limulus test. Crystallization : A recognized method for purification as demonstrated by HPLC Extraction from Proteins Hydrolysates7/8 REXIM S.A.

  11. Biological Safety TSE Expert reports from Pasteur Institute since 1992 Viral Safety Validation report (Pasteur Institute /1998 ) using : Encephalomyocarditis virus Canine Parvovirus classified as « resistant » and « highly resistant » by the CPMP (Committee for Proprietary Medicinal Products ) Extraction from Proteins Hydrolysates8/8 REXIM S.A.

  12. Typical examples : asp ,glu , gly ,lys Process uses activated charcoal,ultrafiltration and Recrystallization Origins: asp : bioconversion of fumaric acid glu , lys : fermentation gly : synthesis Purification from lower Grade ( feed ) REXIM S.A.

  13. Enzymatic resolution at KonstanzL- Methionine1.DL- Methionine -> N-acetyl-DL-Methionine2.acylase-> L-met + N-acetyl-D-Met3.ion exchange separation-> L- Methionine-> N-acetyl-D-Met 4. -> N-acetyl-DL-Methionine REXIM S.A.

  14. Fumaric acid + NH3 + fumarase L-aspartic acid Bioreaction, filtration, precipitation , filtration, drying L-aspartic + aspartate bdecarboxylaseL-alanine Bioreaction, filtration, precipitation , filtration, drying Biocatalysis in China REXIM S.A.

  15. Sugar + Cornsteep + NH3 L- Valine Biomass removal, ion exchange ,crystallisation ,filtration, drying Sugar + Cornsteep + NH3 L- Isoleucine Biomass removal, ion exchange ,crystallisation ,filtration, drying Fermentation in China REXIM S.A.

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