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DNA Isolation. 1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water. Pour liquid through sieve. 3. Add, stir gently, and let sit for 10 min: 2 tablespoons detergent 1/8 teaspoon meat tenderizer .
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DNA Isolation 1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: • 2 tablespoons detergent 1/8 teaspoon meat tenderizer Pour off small amount into tube and gently add equal volume isopropanol to form a layer. DNA is visible where layers meet
DNA Isolation 1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water
DNA Isolation 1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water Pour liquid through sieve
1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: • 2 tablespoons detergent 1/8 teaspoon meat tenderizer DNA Isolation
1. Blend for 15-30 seconds: 100 ml of peas 1/8 teaspoon salt 200 ml cold water Pour liquid through sieve 3. Add, stir gently, and let sit for 10 min: • 2 tablespoons detergent 1/8 teaspoon meat tenderizer Pour off small amount into tube and gently add equal volume isopropanol to form a layer. DNA is visible where layers meet DNA Isolation