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A DNA minor groove electronegative potential genome map based on photo-chemical probing.

A DNA minor groove electronegative potential genome map based on photo-chemical probing. Lindemose S, Nielsen PE, Hansen M, Møllegaard NE. Nucleic Acids Res. 2011 Apr 8. A im. Predicting DNA minor groove electronegative potential based on sequences. Uranyl Photocleavage.

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A DNA minor groove electronegative potential genome map based on photo-chemical probing.

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  1. A DNA minor groove electronegative potential genome map based on photo-chemical probing. Lindemose S, Nielsen PE, Hansen M, Møllegaard NE. Nucleic Acids Res. 2011 Apr 8.

  2. Aim • Predicting DNA minor groove electronegative potential based on sequences.

  3. Uranyl Photocleavage • Sequence dependent cleavage of DNA. • Maximum cleavage correlates with minimum minor groove width and also with the largest electronegative potential.

  4. Material and Methods Pentamer library: 7 members (94 nt each) All possible 1024 combinations of pentanucleotidesequences.

  5. Material and Methods • Sliding window of five bases:

  6. Results

  7. Results

  8. Nucleosome Electrostatic potential in the nucleosome The role of DNA shape in protein-DNA recognition. Rohs R, West SM, Sosinsky A, Liu P, Mann RS, Honig B. Nature. 2009 Oct 29;461(7268):1248-53.

  9. Nucleosome High cleavage in nucleosome, low in promoters.

  10. Sum • Cleavage prediction correlate well with experimental cleavage. • A DNA minor groove electronegative potential genome map based on photo-chemical probing.

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