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Exploring Chromatography Techniques for Analyzing Dyes and Components

Discover various chromatography methods used to analyze dyes in paints, components of crude oil, pesticides in foods, and proteins in blood, following practical activities. Learn to separate mixtures into pure substances and extract useful materials from natural resources. Explore paper chromatography, column chromatography, flash chromatography, and son of chromatography with relevant examples like food dye chromatography and plant pigment chromatography.

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Exploring Chromatography Techniques for Analyzing Dyes and Components

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  1. Son of Flash Chromatography

  2. How would you analyse these? Dyes in paints. Components of crude oil. Pesticides in foods. Proteins in blood.

  3. Chromatography I have participated in practical activities to separate simple mixtures of substances and can relate my findings to my everyday experience. SCN 2-16a I can differentiate between pure substances and mixtures in common use and can select appropriate physical methods for separating mixtures into their components. SCN 3-16a I can participate in practical activities to extract useful substances from natural resources. SCN 3-17b

  4. Chromatography • The mixture is dissolved in a fluid called the "mobile phase“. • This carries it through a structure holding another material called the "stationary phase". • The various constituents of the mixture travel at different speeds, causing them to separate.

  5. Paper Chromatography

  6. Column Chromatography

  7. Flash Chromatography

  8. Flash Chromatography Son of

  9. Chromatography • Use the sheets provided to build it your column and then run it to separate the dyes

  10. Food Dye Chromatography Just in case your experiment did not work properly here is a photo of the separated dyes. E104 Yellow Quinoline Yellow E142 Blue Green S E122 Red Carmoisine

  11. Plant Pigment Chromatography Just in case your experiment did not work properly here is a photo of the separated dyes. Carotene Chlorophyll a Chlorophyll b

  12. Anthocyanin Chromatography Just in case your experiment did not work properly here is a photo of the separated dyes. Chlorophylls Anthocyanins

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