1 / 16

Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid. Bryan Addeman April 6, 2010. Agenda. Background Theory Motivation Measurements Moving Forward. Why use 13 C instead of 1 H?. Label specific metabolites with 13 C 12 C isotope is not magnetically active.

bonita
Download Presentation

Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Effects of low magnetic fields on T1values for hyperpolarized 13C Pyruvic Acid Bryan Addeman April 6, 2010

  2. Agenda • Background Theory • Motivation • Measurements • Moving Forward

  3. Why use 13C instead of 1H? • Label specific metabolites with 13C • 12C isotope is not magnetically active

  4. What is hyperpolarization?

  5. Why does the sample need to be hyperpolarized? • Natural abundance of 13C is ≈ 1.11% • Amount of signal is related to gyromagnetic ratio (γ) & abundance • γH = 42.58 (Mhz/T) • γ13C = 10.71 (Mhz/T) • Hyperpolarization can give a factor of 10,000x more initial signal

  6. Hyperpolarization Process • Inject sample mixed with free radical • Cool to 1.4 K in a High magnetic field • Bombard sample with microwaves • Mix with heated solution • Duration: 1.5 hours • Amount of Hyperpolarization decays with time (T1) Robarts DNP Hyperpolarizer

  7. The T1 Relaxation Process T2 T1 Spin-Spin Relaxation Spin-Lattice Relaxation

  8. Pyruvic Acid

  9. Effects of low magnetic fields on T1values for hyperpolarized 13C Pyruvic Acid

  10. Motivation • Hyperpolarized sample transport: • Bo: High (DNP) -> Low -> High (MRI) • What happens to the sample in transit? • T1 relaxation is a characteristic of the molecule that can be measured

  11. Measuring T1 • Variable magnetic field • Bo = 0.0-0.9 T • Desktop NMR system • Bo = 0.55 T • Both devices can measure Signal amplitude at time intervals Spinmaster Oxford Instruments MQC

  12. Analysis • Fit Equation • Non-Linear Least Squares Fit Bo=0.55, 13C enriched Pyruvic Acid

  13. Results

  14. Conclusions • Need to take more measurements to understand the effects low field strengths on T1 values

  15. Acknowledgments Supervisors • Dr. Charles McKenzie • Dr. Lanette Friesen-Walder • Dr. Tim Scholl • Dr. Jake Onwuachi Lab • Curtis Wiens • Samantha Flood • Steven Yange • Elmer Soto • Trevor Wade

  16. Questions

More Related