Forest Tree § Shrub Seed Committee 2007-2010

1. Day, 19 June 2010 Agenda Point (10:30-11:30) Forest Tree § Shrub Seed Committee2007-2010 Activities 2007-2010 Proposed tasks 2010-2013 prepared by Zdenka Procházková presented by Robert Paul Karrfalt

2. Day, 19 June 2010 Agenda Point (10:30-11:30) Accredited ISTA lab Non ISTA lab ISTA lab

3. Day, 19 June 2010 Agenda Point (10:30-11:30) Planed activities2007-2010 Rules Development Publications Workshop and Seminar Proficiency tests

4. Day, 19 June 2010 Agenda Point (10:30-11:30) A Rules Development A.1 Introduction of New Methods Comparative testsof Abies germination Comparative tetrazolium testsof Abies A.2 Introduction of New Species Tropical tree seeds (germination)

5. Day, 19 June 2010 Agenda Point (10:30-11:30) A1 Introduction of New Methods Comparative testsof Abies germination Goal to evaluate a new dormancy-breaking stratification-redry method in fir seeds (controlling seed moisture content during stratification=prechilling) to compare with current prechilling prescriptions and TEZ test Why to provide customers with better information about seed lot quality and, if it works, then to introduce it as an alternative GER method into the ISTA Rules Responsible V. Vankus ( + G. Edwards,a former FTS member participate in a project on this subject supported by the AOSA )

6. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative testsof Abies germination In planning process …. • Survey of priorities and/or needs of FTS members regarding current Abies germination methods • Use results from Abies alba comparative test on TEZ and germination to assess degree of uniformity in testing between laboratories and for developing comparative germination tests on additional Abies species

7. Day, 19 June 2010 Agenda Point (10:30-11:30) A1 Introduction of New Methods Comparative tetrazolium testsof Abies Goal to compare preparation of wet and dry seeds with either transverse or longitudinal cut method Why to improve specifications related to alternative methods getting more data from accredited (also non-accredited) laboratories and monitoring results achieved by “different” methods Responsible Z. Prochazkova

8. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies Methods used by laboratories

9. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies Seeds -ITML0601, Laboratorio Analisi Sementi Forestali, Peri, Italy 19 labs participated (7 ISTA accredited/ 12 volunteers) 15 countries (AUT, CAN, CZ, DE, FI, FR, HR, HU, IT, LAT, LIT, POL, SK, SPA, USA) 9 labs no experience with Abies (5 ISTA labs)

10. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies

11. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies Results From All Participants Jean-Luis Laffont, chair STO

12. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies ResultsFrom the ISTA Accredited Labs

13. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies Results by Individual Lab, All Labs Included Accredited ISTA lab Wet / Dry methods

14. Day, 19 June 2010 Agenda Point (10:30-11:30) Comparative tetrazolium testsof Abies Conclusion No significant difference between „wet“ / „dry“ methods in labs experienced in TEZ testing of Abies alba Laboratories without any experience have difficulties in seed preparation (transversal opening of embryo cavity to enable sufficient penetration of tetrazolium solution) that ends in improper staining and viability underestimating when strictly followed the ISTA Rules Workshops / training courses / comparative tests are necessary to improve uniformity in TEZ testing of dormant tree seeds

15. Day, 19 June 2010 Agenda Point (10:30-11:30) A2 Introduction of New Species Tropical Tree Seeds Goal to develop germination protocols for tropical tree seeds not included in the ISTA Rules Why to provide internationally recognised methods especiallyfor commercially important tree species Responsible Hugh Pritchard

16. Day, 19 June 2010 Agenda Point (10:30-11:30) Tropical Tree Seeds Germination protocol for seeds of Jatrophacurcas (An oil seed crop for dry poor soils) Charlotte Seal, Suzy Wood, Hugh Pritchard * Germination at 30o C gave greener healthier looking seedlings

17. Day, 19 June 2010 Agenda Point (10:30-11:30) Jatrophacurcas WHAT ELSE IS KNOWN? PSD not defined yet 1000 Seed Weight= 531g (RBG Kew) 2500 seeds (Working sample) = 1330 g Submitted sample = 2660 g Germination The seeds germinate in 7-14 days in organic soil mix with good moisture. The high quality viable seeds have germination rate ≥90%. The (orthodox) seeds stay viable for up to 12 months (air-tight containers, MC 5-7%) but the germination rate may drop to 80% after 6 months. Viability – Kak, A. et al. Assessment of viability of Jatrophacurcas L. seeds using the tetrazolium test, SST 37 (2009) TEZ Committee- developing a procedure

18. Day, 19 June 2010 Agenda Point (10:30-11:30) Tropical Tree Seeds Germination protocol for palmseeds All seeds tested at 30oC on agar. Charlotte Seal, Suzy Wood, Hugh Pritchard

19. Day, 19 June 2010 Agenda Point (10:30-11:30) Tropical Tree Seeds Conclusion Basic germination protocols for many tropical tree species are available Future For introduction in the ISTA Rules - validation of „selected“ methods / species

20. Day, 19 June 2010 Agenda Point (10:30-11:30) BPublications ISTA Tree and Shrub Seed Handbook – revision No progress in last three years… However, as there is interest among the FTS members, this task will be included in the 2010-2013 agenda Why ? To provide laboratories with updated and compiled information focused on the testing of tree and shrub seeds so that more laboratories can correctly apply the ISTA rules. How ? To update the old version (1991) or To prepare a completely new handbook (as the “Flower seed handbook”-sheet for each species)

21. Day, 19 June 2010 Agenda Point (10:30-11:30) BPublications Propágulos e Plântulas da Amazonia. Camargo, J. L. C.; I. D. K. Ferraz, M. R. Mesquita, B. S. Almeida, H. Brum.2008. Volume 1. ISBN 978-85-211-0041-6.Editora INPA, Manaus. 168p.

22. Day, 19 June 2010 Agenda Point (10:30-11:30) CWorkshop and Seminar Planned C.1 Training and education Temperate Tree and Shrub Seed Workshop, Italy Tropical Tree and Shrub Seed Workshop, Brazil C.2 Seminars Trees, Seeds and a Changing Climate, UK

23. Day, 19 June 2010 Agenda Point (10:30-11:30) C.1 Training and education 5th Tree and Shrub Seed Workshop, Peri, Italy 12-14 June 2008 20 participants from 13 countries

24. Day, 19 June 2010 Agenda Point (10:30-11:30) TEZ test(S. Krämer) theoretical aspects, practical preparation and evaluation of Abies alba,Acer pseudoplatanus,Carpinus betulus and Crataegus monogyna seeds

25. Day, 19 June 2010 Agenda Point (10:30-11:30) Sampling(L. Pietilä) theoretical aspects + practical demonstration of Acer pseudoplatanus, Abies alba,Carpinus betulus and Crataegus monogynaseeds sample for moisture content determination; sample reduction

26. Day, 19 June 2010 Agenda Point (10:30-11:30) Oral presentations (9) FTS activities 2001-2008(Z. Prochazkova, F. Gorian) Desiccation tolerance in seed of the New Zealand Flora(C. McGill) Oak testing in Austria(I. Strohschneider) Discussion on Jatrophacurcas(F. Gorian) TEZ testing on dormant California native shrubs(M.Aberle)

27. Day, 19 June 2010 Agenda Point (10:30-11:30) Oral presentations(cont.) MOI test: theoretical and practical aspects (S. Pasquini) MOI test: quality assurance and ISTA MOI Committee (C. McGill) Measurement of water activity on forest tree seed (F. Colas / P. Baldet) Discussion on moisture sample sizes(Z.Prochazkova)

28. Day, 19 June 2010 Agenda Point (10:30-11:30) Field trip at CNBF Peri

29. Day, 19 June 2010 Agenda Point (10:30-11:30) C.1 Training and education Tropical Tree and Shrub Seed Workshop, Brazil Cancelled…

30. Day, 19 June 2010 Agenda Point (10:30-11:30) C.2Seminar Trees, Seeds and a Changing Climate22 – 25 September 2008Organised by: Hugh W. Pritchard, Matt Daws, Kate Hardwick (RBG Kew)40 delegates from Australia, Brazil, Canada, China, Denmark, Finland, Greece, Italy, Poland, RSA, Taiwan, UKMain sponsors: Supported by: ISTA, Botanic Gardens Conservation International (BGCI)

31. Day, 19 June 2010 Agenda Point (10:30-11:30) ProgrammeCLIMATE CHANGE: PREDICTED AND OBSERVED IMPACTSFOREST FRAGMENTATION AND RESTORATIONTREE SEED SCIENCE22 papers and 2 posters Guided tours of the Welcome Trust Millennium Building. A chance to look behind the scenes at the science and facilities of the Seed Conservation Department, including the world-leading conservation work of the Millennium Seed Bank Project

32. Day, 19 June 2010 Agenda Point (10:30-11:30) C.2Seminar OECD Forest Scheme Meetings 2008, 2009 Fabio Gorian

33. Day, 19 June 2010 Agenda Point (10:30-11:30) OECD Forest Scheme Meetings Agenda of the OECD Meeting • Develop a relevant and more up-to-date database • Develop common definitions for terms not used directly in OECD. • Adaptation of the OECD forest scheme to the tropical countries • Short rotation bioenergy plantations and agro-forestry species • Proposal on how to deal with the category “Qualified” • Presentation of the FTSS TCOM decisions • Evaluation of accession proposals by new countries Forest Scheme • Update on both statistics and implementation of the 2007 Forest Scheme in members countries

34. Day, 19 June 2010 Agenda Point (10:30-11:30) OECD Forest Scheme Meetings Development of multilingual forestry dictionary

35. Day, 19 June 2010 Agenda Point (10:30-11:30) DProficiency tests Plan The preliminary round in 2009 Pinus sylvestris purity, 1000 weight, germination

36. Day, 19 June 2010 Agenda Point (10:30-11:30) DProficiency tests Plan The preliminary round in 2009 Pinus sylvestris purity, 1000 weight, germination Postpone

37. Day, 19 June 2010 Agenda Point (10:30-11:30) Proposed tasks 2010-2013 Membership 2010-2013 • Rules Development • Publications • Workshop and Seminar • Proficiency tests

38. Day, 19 June 2010 Agenda Point (10:30-11:30) Membership 2010-2013 New chair Z. Prochazkova has chaired since 2001 2001-2004 2004-2007 2007-2010 TIME FOR CHANGE!

39. Day, 19 June 2010 Agenda Point (10:30-11:30) Membership 2010-2013 YES

40. Day, 19 June 2010 Agenda Point (10:30-11:30) Membership FTS representatives in other technical committees Fabio Gorian STO Robert P. Karrfalt MOI Steffi Krämer TEZ Isolde Ferraz STO Hugh Pritchard STO

41. Day, 19 June 2010 Agenda Point (10:30-11:30) A Rules Development Suggested topics Abies stratification MOI determination (e.g.large tropical seeds) Water activity measurement TEZ (new species) Standardisation (validation) of germination methods for tropical /subtropical tree species Alternative germination substrates (agar??) – for conservation programs (seed banks) – small seed lots

42. Day, 19 June 2010 Agenda Point (10:30-11:30) B Publications Suggested topics Tree and Shrub Seed Handbook revision Isolde D. K. Ferraz Guia de Propágulos e Plântulas da Amazônia, volume 1 - to publish in English Guide to Propagules and Seedlings of Tree Species form the Amazon( Guia de Propágulos e Plântulas da Amazônia),to elaborate volume 2

43. Day, 19 June 2010 Agenda Point (10:30-11:30) C Workshop and Seminar Suggested topics Water activity workshop, 13 – 15 October 2010, Montargis, France Patrick Baldet (FR), Fabienne Colas (CA) in cooperation with FTS +MOI

44. Day, 19 June 2010 Agenda Point (10:30-11:30) D Proficiency tests Suggested topics Pinus sylvestris - PUR, TSW, GER High interest of labs in “comparative“ tests

45. Day, 19 June 2010 Agenda Point (10:30-11:30) Expectations Very low international trade with temperateforest tree seeds (tropical????) Increase /LOSSof ISTA accredited labs testing only forest tree seeds?? Interest from conservation area (seed banks) for internationally recognised (ISTA) methods?

46. Day, 19 June 2010 Agenda Point (10:30-11:30) THANKS My whole-hearted thanks for cooperation and support to all FTS members ISTA secretariat other TCOMs The committee gives its Whole-hearted thanks to Zadenka for 9 of service as chairperson.