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Evolving Resistance: Research Goals and Goods

Evolving Resistance: Research Goals and Goods. Georgiana May Brett Couch Ethy Cannon University of Minnesota. Education Training. Evolution. Map. Resistance Resources. Chromosome 11S. Ever since Grube et al. I2. R3,7. RB. Tomato Potato S. dem. Eggplant Pepper Tobacco.

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Evolving Resistance: Research Goals and Goods

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  1. Evolving Resistance:Research Goals and Goods Georgiana May Brett Couch Ethy Cannon University of Minnesota

  2. Education Training Evolution Map Resistance Resources

  3. Chromosome 11S Ever since Grube et al. I2 R3,7 RB

  4. Tomato Potato S. dem. Eggplant Pepper Tobacco I2- homologous sequences in Solanaceae Couch et al. MPMI 2006

  5. I2- h NBS sequences under positive selection Positive Selection

  6. NBS LRR NBS and LRR regions reassociate through time

  7. Contributions and resources • Birth-death model applies to R gene evolution • 232 sequences from 6 species • positive selection • evolving function? • seek new resistance function in fewer than 10 lineages • Brett Couch • David Schladt

  8. I2 lineages maintained 12 million years Ancient duplications

  9. Contributions and resources • Long term maintenance of R gene lineages - 12 my • 232 sequences from 6 species • maintained through speciation • maintain function? • seek non-host R in fewer than 10 lineages • Brett Couch

  10. I2-h map tochromosomes 8,9,11in tomato 8 11 9 B B B B B IL Block BBAC contig

  11. 250 tomato BAC clones hybridized to I2 probes Oligo probes hybridize to specifc I2 homologs

  12. Map I2-containing BAC clonesto IL segment PCR length polymorphisms in parent lines BAC end polymorphisms segregate in the L. pinnellii mapping population

  13. Contributions and resources • Verified Pan, Fluhr results • chromosomes 8,9,11 • not on 3,4 (Pan) • New region - 9 I • Ancient chromosomal duplications carry R gene duplications • Brett Couch • Deren Eaton

  14. R Baumgarten et al. 2002

  15. Goods solevol demo site Ethalinda Cannon

  16. examples

  17. Resistance Resources • 75 distinct I2-h lineages • over 6 species • http://solevol.ccgb.umn.edu/ • Genome locations • Development of evolutionary models • Training

  18. Resistance Resources Education Training Evolution Map

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