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Ex. 18 Temperature. Temperature Classifications. 1. Psychrophiles 2. Mesophiles 3. Thermophiles. “Cold loving” 0 – 20 degrees C “moderate temp loving” 20 – 40 degrees C “heat loving” 40 – 100 degrees C. Temperature Classifications. 4 c. 37 c. 55 c.
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Temperature Classifications • 1. Psychrophiles • 2. Mesophiles • 3. Thermophiles • “Cold loving” • 0 – 20 degrees C • “moderate temp loving” • 20 – 40 degrees C • “heat loving” • 40 – 100 degrees C
Temperature Classifications 4 c 37 c 55 c
Thermal Death Point (TDP) • Temperature in which all bacteria and their endospores (if they have them) are killed within 10 minutes.
Add .5ml bacteria to Test Tubes 40 60 80 100 40 60 80 100 E. coli Staph. epi 40 60 80 100 40 60 80 100 Micrococcus luteus Bacillus stearothermophilus
Set Hot Plates at desired temperatures 40 60 80 100
40 60 80 100 E. coli Staph. epi Micrococcus luteus Bacillus stearothermophilus