150 likes | 1.12k Views
Plant water regime. Introduction Adaptation, acclimation Poikilohydric and homoiohydric plants Water content, relative water content Water potential and its components Water and growth. Basic characteristics. Water content WC [%] = (FM - DM / FM) 100 FM - fresh mass, DM - dry mass
E N D
Plant water regime • Introduction • Adaptation, acclimation • Poikilohydric and homoiohydric plants • Water content, relative water content • Water potential and its components • Water and growth
Basic characteristics • Water content • WC [%] = (FM - DM / FM) 100 • FM - fresh mass, DM - dry mass • cytoplasm 85 - 90 %, chloroplasts, mitochondria about 50 %, • ripe fruits 85 - 95 %, leaves 80 - 90 %, roots 70 - 95 %, wood 50 %, seeds , pollen grains 5 - 15 % • Relative water content • RWC [%] = [ 1 - (FMs - FMa)/(FMs - DM) ] 100, • FMs- mass after saturation, FMa- actual fresh mass • Plant water balance = absorption - transpiration • Effects of soil moisture and relative humidity
Water potential and its components • w = (w -w0) / Vw • w - water potential, w - chemical potential of water in the system, • w0 - chemical potential of pure water, Vw-- volume per mole of water • = (G / ni)T,p,nj • G - Gibbs free energy, ni- number of moles of component i, nj - number of molesof other components, T - absolute temperature, p - pressure • w = s + p + m + g • s - osmotic potential, p - pressure potential, m - matric potential, g - gravitational potential • s = (RT / Vw) ln aw = -cRT • R - gas constant, aw- water activity, c - concentration • = V (p / V) • - volumetric modulus of elasticity , V - cell volume • Differences in w and its components inside cells and in apoplast (wall a tissue)
Methods of determinationw - psychrometric methods, pressure bomb s - psychrometric and cryoscopic methodsp - pressure probeThermocouple psychrometer
Hofler diagram • The roles of individual components: • g - high trees • m - imbibition and germination (till water content about 60 %) • p - veins, apoplast, growth, movements of stomata • s - transport on cell and tissue level, plasmolysis, plasmoptysis, root pressure, osmotic adjustment, growth uplatnění
Osmotic and elastic adjustment • Osmotic and elastic adjustment (growth, drought, salinity, low temperature, etc.), induction by decrease in soil water potential, air humidity, etc. • Osmotic adjustment – ion uptake, production and accumulation of osmotically active substances such as sugars (glucose, trehalose, saccharose), sugar alcohols (mannitol, sorbitol, glycerol), polyamines, aminoacids (proline), betaines (glycinebetain) • Membrane protection, source of C or N, defence against reactive oxygen species (ROS) • Dehydrines –ripenning of seeds or pollen grains, in plant vegetative parts during stresses, induced also by abscisic acid (ABA) • Elastic adjustment – expansin, endoglucanase, transglycosylase, peroxidase
Changes in water potential and its components during water stress
Water and growth • Elongation: • dV/Vdt m(p - Y) • m - cell wall extensibility, p - pressure potential, Y - threshold pressure potential • dV/Vdt Lp(wo - w) • Lp - hydraulic conductance, wo - water potential of water source, w- water potential of elongating cell • Lockart equation: • dV/Vdt = mLp/(m + Lp) (w + p - Y)
Model of cell wall and possible changes of its extensibility
Comparison of shoot and root growth rate as affected by water potential of substrate
Water stress and growth • Elongation rate (a) and pressure potential (b) in different distance from root apex in two substrates of different water potential