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生物結構期末報告

生物結構期末報告. 學生 : 葉雅如 M93360018 老師 : 鄒文雄 教授. Aim. 分析其他蛋白質與 p25/26 是否有保留性序列存在 , 是否有演化上的關係存在 其他蛋白質中包含 p25/26 相似的序列時 , 是否會造成該蛋白質立體結構改變. Introduction. CFTR , A chloride channel. The cystic fibrosis transmembrane conductance regulator (CFTR) is a 1480 amino acid membrane bound glycoprotein

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生物結構期末報告

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  1. 生物結構期末報告 學生:葉雅如 M93360018 老師: 鄒文雄 教授

  2. Aim • 分析其他蛋白質與p25/26是否有保留性序列存在,是否有演化上的關係存在 • 其他蛋白質中包含p25/26相似的序列時,是否會造成該蛋白質立體結構改變

  3. Introduction • CFTR , A chloride channel • The cystic fibrosis transmembrane conductance regulator (CFTR) is a 1480 amino acid membrane bound • glycoprotein • ABC-ATPase proteins. • two, six span membrane bound regions(TMS1,TMS2), ATP binding domain. • The R-domain is a unique feature of CFTR within the ABC superfamily. Biochemistry,38 (23), 7453 -7461, 1999. 10.1021/bi9903603 S0006-2960(99)00360-8

  4. P26/P25 • 1CKY • Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P26, F508 Mutation Region; Synonym: P26 In H2O; Engineered: Yes • 1CKZ • Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P25, F508 Mutation Region; Synonym: P25 In H2O; Engineered: Yes

  5. NBD1 • 1XMJ • Compound: Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator; Chain: A; Fragment: Nucleotide Binding Domain One; Synonym: Cftr; Camp- Dependent Chloride Channel; Ec: 3.6.3.49; Engineered: Yes; Mutation: Yes

  6. 利用NBD1作protein and protein BLAST

  7. 任選出四條不同E-value序列 • >gi|15889958|ref|NP_355639.1| hypothetical protein AGR_C_4902 [Agrobacterium tumefaciens str. C58] • >gi|15988440|pdb|1JSQ|A Chain A, Structure Of Msba From Escherichia Coli: A Homolog Of The Multidrug Resistance Atp Binding Cas • >gi|15890885|ref|NP_356557.1| hypothetical protein AGR_L_1532 [Agrobacterium tumefaciens str. C58] • >gi|16264640|ref|NP_437432.1| putative ABC transporter protein, fused ATP-binding and membrane-spanning components, secretes ExpE1 [Sinorhizobium meliloti 1021]

  8. Scores Table (7個序列)

  9. Alignment

  10. 討論 • 由於P25/26序列太短無法直接作protein-protein BLAST • 使用NBD1(P25)作搜尋不易找出於與P25/26相對位置相同的protein 故無法做進一步探討 • 但與NBD1相似性高的序列,多屬於各種ATPase-family的膜蛋白,似乎也可推知膜蛋白的具有Domain彼此具有相當的同源性

  11. THE END

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