семинары “molecular biology of the cell”

семинары “molecular biology of the cell” Федор Северин, корпус А, комн. 220 тел. 939-31-07 8-915-120-7955 E-mail: severin@belozersky.msu.ru Святослав Соколов, Дмитрий Кнорре http://yeastmolbiol.belozersky.msu.ru

- why what you are studying is interesting/important

- why what you are studying is interesting/important - what is already known

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • - motivation of a particular experiment

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions

- why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions • General conclusions, models, etc.

motivation of a particular experiment • general experimental setup • specific experiment • Conclusions • Scc1’s cleavage and dissociation from chromosomes • depends both in vitro and in vivo on a protein called • Esp1. Previous studies have left unanswered whether separin • is itself the protease that cleaves Scc1 • To establish whether Scc1 can serve as a cleavage sub • strate whwe purified Scc1 from insect cells exp • pressing a Flag-epitope-tagged yeast SCC1 gene from • a recombinant baculovirus. • all Scc1-P was cleaved by extracts containing • intermediate levels of Esp1 separin (Figure 2B) • Thus, Esp1 separin might indeed be the protease that cleaves Scc1. However, we cannot elimiinnate at this stage the possibility that the Scc1 cleavage • activity is due to a substoichiometric protease that copurifies with separin. Uhlmann et al., Cell, 2000

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семинары “molecular biology of the cell”